Starting from Unite_Euka_8.0_18112018 (cf http://genoweb.toulouse.inra.fr/frogs_databanks/assignation/Unite/Unite_Euka_8.0_18112018.tar.gz ), 
ITS1 and ITS2 are splited and store in 2 different files using ITSx_1.0.10.

Command line
ITSx -i Unite_Euka_8.0_18112018.fasta --cpu 10 --complement F --preserve T  --concat F --table T --graph_scale 1