Starting from UNITE_s_7.1_20112016 (cf http://genoweb.toulouse.inra.fr/frogs_databanks/assignation/Unite/Unite_s_7.1_20112016_ITS.tar.gz ), ITS1 and ITS2 are splited and store in 2 different files using ITSx_1.0.10. Command line ITSx -i Unite_s_7.1_20112016_ITS.fasta -o Unite_s_7.1_20112016 --complement F --preserve T --concat F --table T --graph_scale 1