This reference database was construct for a study published in PlosOne : Deciphering intra-species bacterial diversity of meat and seafood spoilage microbiota using gyrB amplicon sequencing: A comparative analysis with 16S rDNA V3-V4 amplicon sequencing (https://doi.org/10.1371/journal.pone.0204629)

Briefly : 
Bacterial genome sequences were downloaded from Ensembl Bacteria (release 43). Then, embl files were parsed with a BioPython library to obtain only sequences of gyrB and parE in FASTA format. To be sure we kept only sequences of interest, we processed all sequences with cutadapt v1.12. If 5' or 3' extremity primers were not found, the sequences were discarded. The resulting 42,169 gyrB and 24,668 parE sequences were combined with another 30,525 sequences extracted from IMG (release 4.3) and with 389 additional sequences from strains and species known to be important in food microbiota that were not represented in Ensembl and IMG [60]. The final database contained 97,747 formated for FROGS affiliation OTU tool (only for blast search, not RDP)

Taxonomies are defined on 8 ranks, you shoudl add "Strand" to the default rank names in FROGS tools.

Warning : for now, we encountered trouble to format it for RDP. In FROGS affiliation tools you will able to only use Blast as affiliation tool.