PHYMYCO-DB is a database designed to help the mycologist community to access to fungal DNA markers data for phylogenetic analyses. Quality checked nucleotide sequences as well as manually cross-checked alignments, are available for each fungal phylum. All candidate SSU rRNA and EF-1-α gene sequences from all fungal phyla are extracted from GenBank (NCBI). Gene sequences are filtered depending on different constraints (length, number of undetermined nucleotides, presence of long gap in the alignments...) and are locally stored in a relational database system. Sequences are regularly cross-checked by experts curators and poor quality sequences are removed from the dataset. How to cite the PHYMYCO-DB ? PHYMYCO-DB: A Curated Database for Analyses of Fungal Diversity and Evolution Stephane Mahe, Marie Duhamel, Thomas Le Calvez, Laetitia Guillot, Ludmila Sarbu, Anthony Bretaudeau, Olivier Collin, Alexis Dufresne, E. Toby Kiers and Philippe Vandenkoornhuyse. PLoS ONE 7(9): e43117. doi:10.1371/journal.pone.004311