[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 444446 sequences (40000140 bp)... [M::process] read 444446 sequences (40000140 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 209844, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 3496, 9735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28513) [M::mem_pestat] mean and std.dev: (4735.46, 4461.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 37902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (468, 479, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (424, 534) [M::mem_pestat] mean and std.dev: (478.95, 15.55) [M::mem_pestat] low and high boundaries for proper pairs: (402, 556) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 2092, 2116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5984) [M::mem_pestat] mean and std.dev: (1403.65, 1129.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7918) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 444446 reads in 14.749 CPU sec, 3.558 real sec [M::process] read 444446 sequences (40000140 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 209694, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 328, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 638) [M::mem_pestat] mean and std.dev: (323.76, 50.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (468, 479, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (424, 534) [M::mem_pestat] mean and std.dev: (478.87, 15.48) [M::mem_pestat] low and high boundaries for proper pairs: (402, 556) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (329, 1911, 2124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5714) [M::mem_pestat] mean and std.dev: (1500.71, 1098.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7509) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 444446 reads in 15.207 CPU sec, 3.660 real sec [M::process] read 444446 sequences (40000140 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 209802, 35, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 371, 9707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28455) [M::mem_pestat] mean and std.dev: (3464.41, 4289.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 37829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (468, 479, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (424, 534) [M::mem_pestat] mean and std.dev: (478.91, 15.48) [M::mem_pestat] low and high boundaries for proper pairs: (402, 556) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (254, 2097, 2124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5864) [M::mem_pestat] mean and std.dev: (1626.00, 1172.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7734) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 444446 reads in 15.112 CPU sec, 3.629 real sec [M::process] read 143414 sequences (12907260 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 209778, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 367, 9711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28475) [M::mem_pestat] mean and std.dev: (2946.28, 4212.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 37857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (468, 479, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (424, 534) [M::mem_pestat] mean and std.dev: (478.90, 15.47) [M::mem_pestat] low and high boundaries for proper pairs: (402, 556) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 2090, 3010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8538) [M::mem_pestat] mean and std.dev: (1658.81, 1300.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (9691, 9724, 9825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9423, 10093) [M::mem_pestat] mean and std.dev: (9760.75, 65.23) [M::mem_pestat] low and high boundaries for proper pairs: (9289, 10227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 444446 reads in 14.682 CPU sec, 3.642 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 67692, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 367, 9682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28402) [M::mem_pestat] mean and std.dev: (3161.70, 4316.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 37762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (468, 478, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 531) [M::mem_pestat] mean and std.dev: (478.68, 15.45) [M::mem_pestat] low and high boundaries for proper pairs: (405, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 143414 reads in 4.987 CPU sec, 1.228 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -R @RG\tID:1\tSM:SRR7062655\tPL:illumina\tLB:SRR7062655\tPU:1 -t4 Gallus_gallus.Gallus_gallus-5.0.dna.toplevel_chr25-26.fa SRR7062655_R1.fastq.gz SRR7062655_R2.fastq.gz [main] Real time: 16.396 sec; CPU: 65.350 sec