[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 444446 sequences (40000140 bp)...
[M::process] read 444446 sequences (40000140 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 210195, 33, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (471, 481, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (433, 528)
[M::mem_pestat] mean and std.dev: (480.57, 13.89)
[M::mem_pestat] low and high boundaries for proper pairs: (414, 547)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (306, 1900, 2088)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5652)
[M::mem_pestat] mean and std.dev: (1510.88, 971.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7434)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (2316, 4676, 9773)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24687)
[M::mem_pestat] mean and std.dev: (5080.00, 3302.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 32144)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 444446 reads in 15.539 CPU sec, 3.959 real sec
[M::process] read 444446 sequences (40000140 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 210254, 19, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (471, 481, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (433, 528)
[M::mem_pestat] mean and std.dev: (480.57, 13.92)
[M::mem_pestat] low and high boundaries for proper pairs: (414, 547)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (469, 2096, 2844)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7594)
[M::mem_pestat] mean and std.dev: (1976.21, 1672.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9969)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 444446 reads in 15.576 CPU sec, 3.738 real sec
[M::process] read 444446 sequences (40000140 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 210191, 34, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (344, 382, 4778)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13646)
[M::mem_pestat] mean and std.dev: (2904.23, 3357.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18080)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (471, 480, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (433, 528)
[M::mem_pestat] mean and std.dev: (480.50, 13.92)
[M::mem_pestat] low and high boundaries for proper pairs: (414, 547)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (294, 1899, 1986)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5370)
[M::mem_pestat] mean and std.dev: (1442.33, 951.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7062)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (3487, 9705, 9805)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22441)
[M::mem_pestat] mean and std.dev: (6714.85, 3343.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 28759)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 444446 reads in 15.774 CPU sec, 3.858 real sec
[M::process] read 210124 sequences (18911160 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 210254, 17, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (350, 417, 3487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9761)
[M::mem_pestat] mean and std.dev: (1276.44, 1368.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12898)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (471, 481, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (433, 528)
[M::mem_pestat] mean and std.dev: (480.62, 13.90)
[M::mem_pestat] low and high boundaries for proper pairs: (414, 547)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1894, 1979, 2117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1448, 2563)
[M::mem_pestat] mean and std.dev: (2008.90, 90.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1225, 2786)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 444446 reads in 15.166 CPU sec, 3.743 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 99388, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (470, 480, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (430, 530)
[M::mem_pestat] mean and std.dev: (480.33, 13.95)
[M::mem_pestat] low and high boundaries for proper pairs: (410, 550)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 210124 reads in 6.798 CPU sec, 1.660 real sec
[main] Version: 0.7.17-r1188
[main] CMD: bwa mem -R @RG\tID:1\tSM:SRR7062654\tPL:illumina\tLB:SRR7062654\tPU:1 -t4 Gallus_gallus.Gallus_gallus-5.0.dna.toplevel_chr25-26.fa SRR7062654_R1.fastq.gz SRR7062654_R2.fastq.gz
[main] Real time: 17.686 sec; CPU: 69.493 sec