Using GATK jar /usr/local/bioinfo/src/GATK/gatk-4.4.0.0/gatk-package-4.4.0.0-local.jar Running: java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -Xmx7g -jar /usr/local/bioinfo/src/GATK/gatk-4.4.0.0/gatk-package-4.4.0.0-local.jar BaseRecalibrator -I SRR7062654.md.bam -O SRR7062654.md.recal.table --tmp-dir . -R Gallus_gallus.Gallus_gallus-5.0.dna.toplevel_chr25-26.fa --known-sites Gallus_gallus_incl_consequences_chr25-26.vcf --verbosity INFO 11:09:32.046 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/usr/local/bioinfo/src/GATK/gatk-4.4.0.0/gatk-package-4.4.0.0-local.jar!/com/intel/gkl/native/libgkl_compression.so 11:09:32.127 INFO BaseRecalibrator - ------------------------------------------------------------ 11:09:32.131 INFO BaseRecalibrator - The Genome Analysis Toolkit (GATK) v4.4.0.0 11:09:32.131 INFO BaseRecalibrator - For support and documentation go to https://software.broadinstitute.org/gatk/ 11:09:32.131 INFO BaseRecalibrator - Executing as formation@n024 on Linux v4.18.0-372.32.1.el8_6.x86_64 amd64 11:09:32.131 INFO BaseRecalibrator - Java runtime: Java HotSpot(TM) 64-Bit Server VM v17.0.6+9-LTS-190 11:09:32.131 INFO BaseRecalibrator - Start Date/Time: November 7, 2023 at 11:09:31 AM CET 11:09:32.132 INFO BaseRecalibrator - ------------------------------------------------------------ 11:09:32.132 INFO BaseRecalibrator - ------------------------------------------------------------ 11:09:32.133 INFO BaseRecalibrator - HTSJDK Version: 3.0.5 11:09:32.147 INFO BaseRecalibrator - Picard Version: 3.0.0 11:09:32.148 INFO BaseRecalibrator - Built for Spark Version: 3.3.1 11:09:32.148 INFO BaseRecalibrator - HTSJDK Defaults.COMPRESSION_LEVEL : 2 11:09:32.149 INFO BaseRecalibrator - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false 11:09:32.150 INFO BaseRecalibrator - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true 11:09:32.150 INFO BaseRecalibrator - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false 11:09:32.150 INFO BaseRecalibrator - Deflater: IntelDeflater 11:09:32.151 INFO BaseRecalibrator - Inflater: IntelInflater 11:09:32.151 INFO BaseRecalibrator - GCS max retries/reopens: 20 11:09:32.151 INFO BaseRecalibrator - Requester pays: disabled 11:09:32.153 INFO BaseRecalibrator - Initializing engine 11:09:32.159 INFO BaseRecalibrator - Shutting down engine [November 7, 2023 at 11:09:32 AM CET] org.broadinstitute.hellbender.tools.walkers.bqsr.BaseRecalibrator done. Elapsed time: 0.00 minutes. Runtime.totalMemory()=162267136 *********************************************************************** A USER ERROR has occurred: Fasta dict file file:///work/user/formation/Formation_Aln-SNP3/Gallus_gallus.Gallus_gallus-5.0.dna.toplevel_chr25-26.dict for reference file:///work/user/formation/Formation_Aln-SNP3/Gallus_gallus.Gallus_gallus-5.0.dna.toplevel_chr25-26.fa does not exist. Please see http://gatkforums.broadinstitute.org/discussion/1601/how-can-i-prepare-a-fasta-file-to-use-as-reference for help creating it. *********************************************************************** Set the system property GATK_STACKTRACE_ON_USER_EXCEPTION (--java-options '-DGATK_STACKTRACE_ON_USER_EXCEPTION=true') to print the stack trace.