Using GATK jar /usr/local/bioinfo/src/GATK/gatk-4.4.0.0/gatk-package-4.4.0.0-local.jar Running: java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -Xmx14g -jar /usr/local/bioinfo/src/GATK/gatk-4.4.0.0/gatk-package-4.4.0.0-local.jar ApplyBQSR -R Gallus_gallus.Gallus_gallus-5.0.dna.toplevel_chr25-26.fa --input SRR7062655.md.bam --output SRR7062655.md.recal.bam --bqsr-recal-file SRR7062655.md.recal.table 11:12:39.374 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/usr/local/bioinfo/src/GATK/gatk-4.4.0.0/gatk-package-4.4.0.0-local.jar!/com/intel/gkl/native/libgkl_compression.so 11:12:39.408 INFO ApplyBQSR - ------------------------------------------------------------ 11:12:39.410 INFO ApplyBQSR - The Genome Analysis Toolkit (GATK) v4.4.0.0 11:12:39.410 INFO ApplyBQSR - For support and documentation go to https://software.broadinstitute.org/gatk/ 11:12:39.410 INFO ApplyBQSR - Executing as formation@n024 on Linux v4.18.0-372.32.1.el8_6.x86_64 amd64 11:12:39.410 INFO ApplyBQSR - Java runtime: Java HotSpot(TM) 64-Bit Server VM v17.0.6+9-LTS-190 11:12:39.411 INFO ApplyBQSR - Start Date/Time: November 7, 2023 at 11:12:39 AM CET 11:12:39.411 INFO ApplyBQSR - ------------------------------------------------------------ 11:12:39.411 INFO ApplyBQSR - ------------------------------------------------------------ 11:12:39.412 INFO ApplyBQSR - HTSJDK Version: 3.0.5 11:12:39.412 INFO ApplyBQSR - Picard Version: 3.0.0 11:12:39.413 INFO ApplyBQSR - Built for Spark Version: 3.3.1 11:12:39.413 INFO ApplyBQSR - HTSJDK Defaults.COMPRESSION_LEVEL : 2 11:12:39.413 INFO ApplyBQSR - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false 11:12:39.414 INFO ApplyBQSR - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true 11:12:39.414 INFO ApplyBQSR - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false 11:12:39.414 INFO ApplyBQSR - Deflater: IntelDeflater 11:12:39.414 INFO ApplyBQSR - Inflater: IntelInflater 11:12:39.415 INFO ApplyBQSR - GCS max retries/reopens: 20 11:12:39.415 INFO ApplyBQSR - Requester pays: disabled 11:12:39.416 INFO ApplyBQSR - Initializing engine 11:12:39.695 INFO ApplyBQSR - Done initializing engine 11:12:39.741 INFO ProgressMeter - Starting traversal 11:12:39.742 INFO ProgressMeter - Current Locus Elapsed Minutes Reads Processed Reads/Minute 11:12:49.760 INFO ProgressMeter - 25:1128114 0.2 139000 833000.4 11:12:59.767 INFO ProgressMeter - 26:690216 0.3 674000 2020181.8 11:13:09.773 INFO ProgressMeter - 26:3337625 0.5 1372000 2741258.7 11:13:19.788 INFO ProgressMeter - 26:5152152 0.7 1882000 2820039.0 11:13:20.538 WARN IntelInflater - Zero Bytes Written : 0 11:13:20.542 INFO ApplyBQSR - 0 read(s) filtered by: WellformedReadFilter 11:13:20.543 INFO ProgressMeter - 26:5311728 0.7 1921919 2826351.5 11:13:20.544 INFO ProgressMeter - Traversal complete. Processed 1921919 total reads in 0.7 minutes. 11:13:20.594 INFO ApplyBQSR - Shutting down engine [November 7, 2023 at 11:13:20 AM CET] org.broadinstitute.hellbender.tools.walkers.bqsr.ApplyBQSR done. Elapsed time: 0.69 minutes. Runtime.totalMemory()=243335168