Using GATK jar /usr/local/bioinfo/src/GATK/gatk-4.4.0.0/gatk-package-4.4.0.0-local.jar Running: java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -Xmx14g -jar /usr/local/bioinfo/src/GATK/gatk-4.4.0.0/gatk-package-4.4.0.0-local.jar ApplyBQSR -R Gallus_gallus.Gallus_gallus-5.0.dna.toplevel_chr25-26.fa --input SRR7062654.md.bam --output SRR7062654.md.recal.bam --bqsr-recal-file SRR7062654.md.recal.table 11:12:38.509 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/usr/local/bioinfo/src/GATK/gatk-4.4.0.0/gatk-package-4.4.0.0-local.jar!/com/intel/gkl/native/libgkl_compression.so 11:12:38.563 INFO ApplyBQSR - ------------------------------------------------------------ 11:12:38.565 INFO ApplyBQSR - The Genome Analysis Toolkit (GATK) v4.4.0.0 11:12:38.566 INFO ApplyBQSR - For support and documentation go to https://software.broadinstitute.org/gatk/ 11:12:38.566 INFO ApplyBQSR - Executing as formation@n002 on Linux v4.18.0-372.32.1.el8_6.x86_64 amd64 11:12:38.566 INFO ApplyBQSR - Java runtime: Java HotSpot(TM) 64-Bit Server VM v17.0.6+9-LTS-190 11:12:38.579 INFO ApplyBQSR - Start Date/Time: November 7, 2023 at 11:12:38 AM CET 11:12:38.579 INFO ApplyBQSR - ------------------------------------------------------------ 11:12:38.579 INFO ApplyBQSR - ------------------------------------------------------------ 11:12:38.580 INFO ApplyBQSR - HTSJDK Version: 3.0.5 11:12:38.580 INFO ApplyBQSR - Picard Version: 3.0.0 11:12:38.581 INFO ApplyBQSR - Built for Spark Version: 3.3.1 11:12:38.581 INFO ApplyBQSR - HTSJDK Defaults.COMPRESSION_LEVEL : 2 11:12:38.582 INFO ApplyBQSR - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false 11:12:38.582 INFO ApplyBQSR - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true 11:12:38.583 INFO ApplyBQSR - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false 11:12:38.583 INFO ApplyBQSR - Deflater: IntelDeflater 11:12:38.583 INFO ApplyBQSR - Inflater: IntelInflater 11:12:38.584 INFO ApplyBQSR - GCS max retries/reopens: 20 11:12:38.584 INFO ApplyBQSR - Requester pays: disabled 11:12:38.585 INFO ApplyBQSR - Initializing engine 11:12:38.753 INFO ApplyBQSR - Done initializing engine 11:12:38.767 INFO ProgressMeter - Starting traversal 11:12:38.768 INFO ProgressMeter - Current Locus Elapsed Minutes Reads Processed Reads/Minute 11:12:48.779 INFO ProgressMeter - 25:2250230 0.2 376000 2254872.6 11:12:58.795 INFO ProgressMeter - 26:2181630 0.3 1079000 3234250.9 11:13:08.793 INFO ProgressMeter - 26:4588744 0.5 1794000 3585131.9 11:13:11.624 WARN IntelInflater - Zero Bytes Written : 0 11:13:11.632 INFO ApplyBQSR - 0 read(s) filtered by: WellformedReadFilter 11:13:11.633 INFO ProgressMeter - 26:5311530 0.5 1988606 3630499.3 11:13:11.633 INFO ProgressMeter - Traversal complete. Processed 1988606 total reads in 0.5 minutes. 11:13:11.684 INFO ApplyBQSR - Shutting down engine [November 7, 2023 at 11:13:11 AM CET] org.broadinstitute.hellbender.tools.walkers.bqsr.ApplyBQSR done. Elapsed time: 0.55 minutes. Runtime.totalMemory()=243335168