FastQCFastQC Report
Mon 14 Mar 2016
MT_rep1_1_Ch6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT_rep1_1_Ch6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624613
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCGC1302.9454054E-421.9195828
CGCGAAT1404.8279765E-420.3727071
CTCGCGA1350.00936073617.6060431
CGGTCGT1350.00941065917.5897882
CGACGAT9300.016.8677251
CTCGAAT8350.016.509621
GACGATC8200.016.2169272
CTCCGTA2355.1029863E-516.1825771
TTCGAGG4652.2191671E-1015.3201389
CTCCGTT4401.6480044E-915.1251931
CCGCTGT5751.8189894E-1214.8671948
CGATCCA10550.014.4052854
GAGTTCG4955.693437E-1014.3916456
CCGGTCG2000.00525253714.2608961
TGTGCCG2000.00528482614.2477297
TCGAATC4704.2327883E-914.1466812
TAATCGA2359.411041E-414.1466814
ATCCGCT6450.013.9900196
TCCGCTG6900.013.7659217
TATCCGC5903.8198777E-1113.6842595
Produced by FastQC (version 0.11.2)