FastQCFastQC Report
Mon 14 Mar 2016
MT_rep1_2_Ch6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT_rep1_2_Ch6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624613
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT1200.00476705519.7897112
CGAGAAC3953.619789E-1016.833781
CGGCAAT2709.490865E-615.8317691
CTCGGTC1800.002620961315.8317691
CTCGGCT2406.155005E-515.8317681
CGCATAT3654.1998646E-815.6148951
CTGTCGG2601.2175495E-414.61484059
CGCAAAC2307.9938956E-414.4550941
CGCAGAT2651.4324341E-414.3382061
CTCGCAG2359.406727E-414.1475381
CGGCAAG2802.2856506E-413.5700881
CGATCGT2450.001288681413.5700881
TCGAATC5051.15960574E-813.1670162
CGAAGAT8350.013.082541
CGACGAT8750.013.0272841
CCGAGAT4505.411948E-712.6654161
CCGAGTT5654.107278E-912.6093741
CGATCCA10250.012.5109594
TCTGTCG3054.7055708E-412.4585528
GACGAGT3054.7080123E-412.4577862
Produced by FastQC (version 0.11.2)