FastQCFastQC Report
Mon 14 Mar 2016
WT_rep1_1_Ch6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_rep1_1_Ch6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340546
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA1350.00940818817.590324
CGACGAT6400.016.3430841
CACGAGT2154.7549672E-415.4792841
CGGGCTT2000.00524662814.2630561
CCGGCTT5752.5465852E-1114.0563441
CTCGGAT3507.1924005E-613.5838621
CGGCTTA4202.3396206E-713.5696762
CTCGCAT3558.326702E-613.392541
GACGATC6501.4551915E-1113.1521462
CGCTAAT2200.00985708612.96641351
CGATCCA7501.8189894E-1212.66503054
TCCGCTG4556.187074E-712.5258547
ACAGCCG3501.09200075E-412.2127086
CACGGAT3156.1230146E-412.0745451
CCGCTGT3551.2465095E-412.0406978
ACGATCC7652.0008883E-1111.7958623
CGCATTT6452.2810127E-911.7937411
TCGCACT3309.1274735E-411.5136649
CGATTTA3309.1274735E-411.5136644
ATGTCGC3350.001035242511.3418186
Produced by FastQC (version 0.11.2)