FastQCFastQC Report
Mon 14 Mar 2016
WT_rep1_2_Ch6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_rep1_2_Ch6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340546
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT1107.016206E-834.5443422
CCGCGCT650.00584533429.2298281
CGGGAAT1751.4869875E-724.4277841
CCGGTCG1601.8604624E-623.7492331
ATCCGCT3404.1836756E-1119.5581956
CTCGGCT1707.957081E-519.5581951
TATCCGC3401.7507773E-816.7641665
CTCGGTT2858.5878855E-716.6661321
GGTCGTC2355.1368148E-516.1696933
CCGCTGT3654.1945896E-815.6159358
CGGGGTT2205.703077E-415.1131511
CCGCATT2205.703077E-415.1131511
CACGGGA2601.2172454E-414.6149141
CGCTGTC4302.0023435E-814.3600039
TCCGCTG4653.6325218E-914.3006157
CGGCCTC2359.400482E-414.148483
CGACTAT2359.400482E-414.148481
CCGGAAT3759.673367E-713.9328851
GTTCGAT5201.1878001E-913.7014821
GATGCGC1050.007296874713.570991582-83
Produced by FastQC (version 0.11.2)