[2016-03-14 17:15:56] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2016-03-14 17:15:56] Checking for Bowtie Bowtie version: 2.1.0.0 [2016-03-14 17:15:56] Checking for Bowtie index files (genome).. [2016-03-14 17:15:56] Checking for reference FASTA file Warning: Could not find FASTA file bowtie2-index/tomato_chr6.fa [2016-03-14 17:15:56] Reconstituting reference FASTA file from Bowtie index Executing: /usr/local/bioinfo/bin/bowtie2-inspect bowtie2-index/tomato_chr6 > aln_tophat_mt/tmp/tomato_chr6.fa [2016-03-14 17:16:01] Generating SAM header for bowtie2-index/tomato_chr6 [2016-03-14 17:16:02] Preparing reads left reads: min. length=101, max. length=101, 1624611 kept reads (2 discarded) right reads: min. length=101, max. length=101, 1624533 kept reads (80 discarded) [2016-03-14 17:17:18] Mapping left_kept_reads to genome tomato_chr6 with Bowtie2 [2016-03-14 17:22:30] Mapping left_kept_reads_seg1 to genome tomato_chr6 with Bowtie2 (1/4) [2016-03-14 17:23:04] Mapping left_kept_reads_seg2 to genome tomato_chr6 with Bowtie2 (2/4) [2016-03-14 17:23:46] Mapping left_kept_reads_seg3 to genome tomato_chr6 with Bowtie2 (3/4) [2016-03-14 17:24:18] Mapping left_kept_reads_seg4 to genome tomato_chr6 with Bowtie2 (4/4) [2016-03-14 17:25:16] Mapping right_kept_reads to genome tomato_chr6 with Bowtie2 [2016-03-14 17:30:33] Mapping right_kept_reads_seg1 to genome tomato_chr6 with Bowtie2 (1/4) [2016-03-14 17:31:16] Mapping right_kept_reads_seg2 to genome tomato_chr6 with Bowtie2 (2/4) [2016-03-14 17:31:54] Mapping right_kept_reads_seg3 to genome tomato_chr6 with Bowtie2 (3/4) [2016-03-14 17:32:33] Mapping right_kept_reads_seg4 to genome tomato_chr6 with Bowtie2 (4/4) [2016-03-14 17:33:36] Searching for junctions via segment mapping [2016-03-14 17:35:36] Retrieving sequences for splices [2016-03-14 17:35:38] Indexing splices [2016-03-14 17:35:49] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/4) [2016-03-14 17:36:17] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/4) [2016-03-14 17:36:43] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/4) [2016-03-14 17:37:09] Mapping left_kept_reads_seg4 to genome segment_juncs with Bowtie2 (4/4) [2016-03-14 17:37:42] Joining segment hits [2016-03-14 17:38:47] Mapping right_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/4) [2016-03-14 17:39:12] Mapping right_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/4) [2016-03-14 17:39:42] Mapping right_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/4) [2016-03-14 17:40:08] Mapping right_kept_reads_seg4 to genome segment_juncs with Bowtie2 (4/4) [2016-03-14 17:40:42] Joining segment hits [2016-03-14 17:41:45] Reporting output tracks ----------------------------------------------- [2016-03-14 17:47:27] A summary of the alignment counts can be found in aln_tophat_mt/align_summary.txt [2016-03-14 17:47:27] Run complete: 00:31:31 elapsed