FastQCFastQC Report
mer. 23 nov. 2011
s_1_uT4.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename s_1_uT4.fastq
File type Conventional base calls
Encoding Illumina 1.5
Total Sequences 45156426
Filtered Sequences 0
Sequence length 36
%GC 48

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA 2235457 4.9504737155238985 Illumina Single End Adapter 2 (95% over 24bp)
GCATGTTTGTGGAGAACCTGGTGCTAAATCACTCGT 1768540 3.9164747006328624 No Hit
GTTCTACAGTCCGACGATCATCTCGTATGCCGTCTT 775515 1.7173967665200078 Illumina PCR Primer Index 7 (95% over 22bp)
GTGGAGAACCTGGTGCTAAATCACTCGTATGCCGTC 681681 1.5095990989189445 No Hit
ATGTTTGTGGAGAACCTGGTGCTAAATCACTCGTAT 594213 1.3158990926341247 No Hit
ACCGCATGTTTGTGGAGAACCTGGTGCTAAATCACT 544349 1.2054740558962749 No Hit
GGAGAACCTGGTGCTAAATCACTCGTATGCCGTCTT 536951 1.189091005563638 No Hit
CAGAGTTCTACAGTCCGACGATCATCTCGTATGCCG 486285 1.0768899203847533 Illumina DpnII expression Sequencing Primer (100% over 23bp)
TAGCTTATCAGACTGGTGTTGGCATCTCGTATGCCG 444401 0.9841367870876229 No Hit
AGAGTTCTACAGTCCGACGATCATCTCGTATGCCGT 439041 0.9722669371575156 Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGTTGGCAGCGGCGACTCTGGACGATCTCGTATGCC 434536 0.9622905054531995 No Hit
TAGCGCCGTGGAGCTTCGGTTGGCCCGGGATAGCCT 420568 0.9313580308592182 No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGATCCAAGC 418781 0.927400676041102 No Hit
AGTTCTACAGTCCGACGATCATCTCGTATGCCGTCT 384872 0.852308373563488 Illumina PCR Primer Index 7 (95% over 21bp)
GAGTTCTACAGTCCGACGATCATCTCGTATGCCGTC 350056 0.7752074975995665 Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATGCGCACCGCATGTTTGTGGAGAACCTGGTGCTAA 349920 0.7749063223028324 No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCT 315579 0.6988573453532394 No Hit
TGTTTGTGGAGAACCTGGTGCTAAATCACTCGTATG 277267 0.6140144926438598 No Hit
CAACTCTTAGCGGTGGATCACTCGGATCTCGTATGC 274815 0.6085844792056838 No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAT 264855 0.5865278177683947 No Hit
CCGCATGTTTGTGGAGAACCTGGTGCTAAATCACTC 253411 0.5611848023579191 No Hit
CAACTCTTAGCGGTGGATCACTCGGCATCTCGTATG 247108 0.5472266560688395 No Hit
TTTGTGGAGAACCTGGTGCTAAATCACTCGTATGCC 247097 0.5472022963021919 No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGTATGCCG 240255 0.5320505214473793 No Hit
TGTGGAGAACCTGGTGCTAAATCACTCGTATGCCGT 237316 0.5255420347039865 No Hit
GTTCAGAGTTCTACAGTCCGACGATCGTATGCCGTC 232374 0.5145978559064883 Illumina DpnII expression Sequencing Primer (100% over 26bp)
CTACAGTCCGACGATCATCTCGTATGCCGTCTTCTG 211831 0.46910488442995907 Illumina PCR Primer Index 7 (96% over 25bp)
GTTCTACAGTCCGACGATCGTTCTACAGTCCGACGA 210758 0.46672869991969695 No Hit
AACTCTTAGCGGTGGATCACTCGGATCTCGTATGCC 203763 0.45123810285605864 No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTATGCCGT 192588 0.42649079446632915 No Hit
TACGACCTCAGATCAGACGAGACAACCCGCTGAATT 190745 0.42240942629073436 No Hit
AACTCTTAGCGGTGGATCACTCGGCATCTCGTATGC 179480 0.3974628107193426 No Hit
TTCTACAGTCCGACGATCATCTCGTATGCCGTCTTC 171111 0.3789294573489939 Illumina PCR Primer Index 7 (95% over 23bp)
TTGTGGAGAACCTGGTGCTAAATCACTCGTATGCCG 170287 0.37710468937466396 No Hit
TGAAAAGAACTTTGAAGAGAGAGTATCTCGTATGCC 162463 0.35977825171549227 No Hit
TCTACAGTCCGACGATCATCTCGTATGCCGTCTTCT 161557 0.35777189275342564 Illumina PCR Primer Index 7 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCATCTCGTA 156722 0.34706466804968134 No Hit
CACCGCATGTTTGTGGAGAACCTGGTGCTAAATCAC 153808 0.3406115444123058 No Hit
GAGAACCTGGTGCTAAATCACTCGTATGCCGTCTTC 150018 0.3322184975400843 No Hit
GTTTGTGGAGAACCTGGTGCTAAATCACTCGTATGC 143942 0.3187630482536417 No Hit
TTTCCGTAGGTGAACCTGCGGAAGGATCATTAATCT 143182 0.31708000983071605 No Hit
CGCATGTTTGTGGAGAACCTGGTGCTAAATCACTCG 139285 0.30845000886474055 No Hit
GTTCTACAGTCCGACGATCGTATGCCGTCTTCTGCT 139175 0.3082064111982644 No Hit
TACGACCTCAGATCAGAATCTCGTATGCCGTCTTCT 135209 0.29942360805968127 RNA PCR Primer, Index 40 (96% over 25bp)
TAGCGCCGTGGAGCTTCATCTCGTATGCCGTCTTCT 132644 0.2937433533823071 RNA PCR Primer, Index 45 (95% over 23bp)
TACGACCTCAGATCAGACGATCTCGTATGCCGTCTT 132568 0.29357504954001457 RNA PCR Primer, Index 22 (95% over 21bp)
TAGCTTATCAGACTGGTGTTGGATCTCGTATGCCGT 132293 0.29296605537382436 No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGTGAGTCG 131979 0.2922706947622471 No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGTCGA 129885 0.2876334810022388 No Hit
GTACAACTCTTAGCGGATCTCGTATGCCGTCTTCTG 127113 0.2814948198070414 RNA PCR Primer, Index 23 (96% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTC 126264 0.2796146887266942 No Hit
TACGACCTCAGATCAGACGAGAATCTCGTATGCCGT 118740 0.26295260833973 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC 115939 0.2567497259415526 Illumina Single End Adapter 2 (95% over 24bp)
TGGAGAACCTGGTGCTAAATCACTCGTATGCCGTCT 115281 0.25529256899117747 No Hit
GAGAGAAATCTGTTGATAACAGAGACTGCATCTCGT 112660 0.24948830095632457 No Hit
GTTGTCGTGGCCGAGTGGTTAAGGCAATGATCTCGT 111731 0.247431007936722 No Hit
GTTGTCGTGGCCGAGTGGTTAAGGCTATGATCTCGT 107806 0.23873900029200718 No Hit
TGAGGTAGTAGATTGAATAGTTATCTCGTATGCCGT 107667 0.23843118142255104 No Hit
AGAACCTGGTGCTAAATCACTCGTATGCCGTCTTCT 94840 0.21002547898719887 No Hit
TACGACCTCAGATCAGACGAGACAACCATCTCGTAT 94676 0.20966229701172542 No Hit
CAACTCTTAGCGGTGGATCACTCGGCTATCTCGTAT 94219 0.20865025943372933 No Hit
TACGACCTCAGATCAGACGAGACAACCCATCTCGTA 93897 0.2079371826282266 No Hit
TCTTCTTATCAGCACACTAGTGCTTTATATCATCGT 90886 0.20126925013950395 No Hit
GTACAACTCTTAGCGGTGGATCACTCGGCATCTCGT 89287 0.19772822587863795 No Hit
TACAGTCCGACGATCATCTCGTATGCCGTCTTCTGC 84011 0.18604439598474867 Illumina PCR Primer Index 7 (96% over 26bp)
GGTTGGCAGCGGCGACTCTGGACGCATCTCGTATGC 79103 0.1751755110114339 No Hit
TACGACCTCAGATCAGACGAATCTCGTATGCCGTCT 78788 0.174477935875616 Illumina PCR Primer Index 4 (95% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATC 76502 0.16941553346139485 No Hit
TCGCCACTGCTGGAAGTTCGTATCTCGTATGCCGTC 69929 0.15485946562732844 No Hit
CAACTCTTAGCGGTGGATCACTCGGCTCGTGATCTC 68607 0.15193186458113403 No Hit
TTCCTATGCATATACCTCTTTATCTCGTATGCCGTC 68462 0.15161075856623374 No Hit
CAGTCCGACGATCATCTCGTATGCCGTCTTCTGCTT 67640 0.14979041964038517 Illumina PCR Primer Index 7 (100% over 27bp)
GGAATTGTGCCTGAATGCTTAAGGACCATCTCGTAT 65909 0.14595707817974787 No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCG 64973 0.14388428349046048 No Hit
CAACTCTTAGCGGATCTCGTATGCCGTCTTCTGCTT 63593 0.14082824003830594 RNA PCR Primer, Index 23 (96% over 28bp)
CATGTTTGTGGAGAACCTGGTGCTAAATCACTCGTA 62969 0.13944637691211434 No Hit
TGAGAACTGAATTCCATAGATGGATCTCGTATGCCG 61931 0.13714770075027638 No Hit
TACGACCTCAGATCAGACGAGACAACCCGCTATCTC 61469 0.1361245905510768 No Hit
TACGACCTCAGATCAGACGAGACAACATCTCGTATG 59651 0.13209858548149936 No Hit
AACTCTTAGCGGATCTCGTATGCCGTCTTCTGCTTG 59422 0.13159145943038095 RNA PCR Primer, Index 23 (96% over 29bp)
TACGACCTCAGATCAGACGAGACATCTCGTATGCCG 59079 0.1308318776158237 No Hit
TGTCCGTCGGCGTCCCGAAGGTGGATCGGTGGGGGT 58661 0.12990620648321458 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA 58115 0.12869707624779694 Illumina Single End Adapter 2 (95% over 24bp)
TGAGGTAGTAGGTTGTATAGTTATCTCGTATGCCGT 57204 0.12667964466452683 No Hit
AAGTTCTACAGTCCGACGATCATCTCGTATGCCGTC 56016 0.12404878986658509 Illumina DpnII expression Sequencing Primer (95% over 21bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG 54104 0.11981461951838261 Illumina Single End Adapter 2 (95% over 24bp)
GTACAACTCTTAGCGGTGGATCATCTCGTATGCCGT 53772 0.11907939747047298 No Hit
TACGACCTCAGATCAGACGAGACAACCCGCTGAATA 53592 0.11868078310714847 No Hit
ACAGTCCGACGATCATCTCGTATGCCGTCTTCTGCT 51401 0.113828760495793 Illumina PCR Primer Index 7 (96% over 27bp)
TGACTGATTTGCATTCGTATGCCGTCTTCTGCTTGA 51032 0.11301160105097778 Illumina Single End Adapter 2 (95% over 22bp)
TAGCGCCGTGGAGCTTCGGTTATCTCGTATGCCGTC 50126 0.11100524208891112 No Hit
TACGACCTCAGAATCTCGTATGCCGTCTTCTGCTTG 49952 0.11061991487103075 RNA PCR Primer, Index 40 (100% over 32bp)
TTTGATTGTGATTTTGAACTTTATAGACAATCTCGT 48600 0.1076258780976156 No Hit
TACGACCTCAGATCATCTCGTATGCCGTCTTCTGCT 48459 0.10731363017967809 Illumina PCR Primer Index 7 (100% over 28bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 23689140 27.793013 108.76543 30
CTCGT 32241370 19.298151 68.79267 32
TCGTA 29023110 18.232994 59.708866 31
ATCTC 26906690 17.711988 58.900467 1
CGTAT 26840270 16.861683 59.558434 32
TCTCG 27513245 16.468122 55.773445 25
ATGCC 19743560 15.0316105 67.59275 8
GTATG 24116580 14.458954 53.64272 6
TATGC 21542800 13.5336895 55.690968 7
TGCCG 17656320 12.222937 61.425865 9
GCCGT 15849035 10.971807 61.194942 10
CCGTC 12824225 9.302493 63.8199 11
AGAAC 8786305 8.930506 63.53186 13
ATCAC 9928540 8.313237 50.464523 28
GAGAA 8454705 8.20116 59.316563 12
CATCT 12101410 7.9660497 28.445507 23
GATCA 9717745 7.765285 62.91021 16
GTGGA 11195100 7.762869 43.38709 9
GAACC 7890440 7.641158 59.166298 14
GACGA 8081120 7.468555 50.56294 16
CACTC 9114050 7.2708373 48.041668 30
GGAGA 7787095 6.8682737 53.981598 11
ACTCG 8991415 6.8455467 46.096634 31
AAATC 7576635 6.6584487 55.05356 26
CGATC 8498150 6.470003 33.64097 15
AACCT 7642345 6.3989906 50.96066 15
TCACT 9718255 6.397279 39.69067 29
CGTCT 10475570 6.2701783 53.136364 12
AATCA 7090155 6.230923 53.27103 27
CTAAA 7042950 6.189439 53.35991 24
ACCTG 7894330 6.010289 46.411182 16
GCTAA 7485175 5.981276 48.482296 23
CCGAC 6061940 5.5931664 40.727554 11
CCTGG 8016250 5.549407 42.585117 17
TGGAG 7894985 5.4745135 42.658836 10
ACGAT 6699975 5.3538365 35.217148 14
CGACG 5975315 5.2615533 38.20923 12
TGGTG 9550465 5.206441 32.733402 19
TAAAT 7071500 5.1279316 44.245037 25
GGATC 6820945 4.9560056 37.552006 15
CTGGT 8655955 4.944522 34.52819 18
GGTGC 7464875 4.931795 39.40563 20
ACAGT 6020015 4.810492 35.977177 6
CTACA 5720270 4.7896233 38.06081 4
TACAG 5981475 4.7796955 36.124084 5
TCCGA 6164925 4.6936193 33.559433 10
CAGTC 6154755 4.685877 34.16053 7
AGTCC 6090875 4.637242 34.118866 8
GTCTT 9301740 4.5941 43.626186 13
TGCTA 7284115 4.576051 37.448288 22
GATCT 7270600 4.5675607 18.782988 24
GTGCT 7588930 4.335008 34.5026 21
GTCCG 6214770 4.302297 30.445915 9
GAAAA 4012250 4.2802544 93.96956 24
AGCGG 4959000 4.1672964 52.77478 8
TGAAA 4889955 4.101178 73.92125 23
TCATC 5958410 3.9222693 19.733244 18
TCTTC 7415240 3.837553 45.34581 14
TCAGA 4747085 3.7933152 53.086166 8
ATCAT 5396935 3.7287736 20.496094 17
TGTGG 6715940 3.6611984 34.218796 8
TCTAC 5399260 3.5541952 29.924767 3
CTTCT 6830480 3.5349267 45.608784 15
AACTC 4180710 3.5005386 41.84138 2
TTGAA 5304030 3.4972918 58.01671 22
GGTGG 5425605 3.4208791 29.793066 12
GCATC 4456030 3.3925653 16.05244 25
TAGCG 4621700 3.3580635 35.99414 8
ACTCT 4945305 3.2553682 33.031544 3
TGGAT 5006210 3.0014439 28.007217 14
TTCTG 6027255 2.9768422 43.703217 16
TCTGC 4948640 2.9620209 52.24885 17
TTGTG 6234590 2.9386725 29.613495 7
TTCTA 5370240 2.9169884 24.929163 2
GCATG 4011455 2.914668 44.505997 1
CTGCT 4701925 2.8143487 52.232407 18
AATCT 3911060 2.702174 9.620127 27
CTTAG 4060055 2.5506215 31.450527 6
GTTCT 5146425 2.5418031 22.299519 1
GTTTG 5334375 2.5143566 28.959269 5
CTCGG 3625630 2.5099137 31.073929 21
ATGTT 4785910 2.480919 31.790527 3
CATGT 3947795 2.480097 38.423653 2
TTTGT 6045385 2.4637363 25.674133 6
TTAGC 3886115 2.441348 31.620825 7
CGGTG 3694735 2.4409885 30.724323 11
CTTGA 3781155 2.37541 54.775352 21
GCGGT 3530360 2.3323913 30.748232 10
TCGGC 3321050 2.2990623 24.528461 22
GCTTG 3967965 2.2666118 50.097633 20
TCTTA 4124750 2.2404675 27.561064 5
CAACT 2674000 2.2389596 42.569557 1
AGAAT 2649985 2.2225277 37.719524 20
AGATC 2775015 2.2174675 35.004436 10
GTAGA 2902155 2.2131948 30.480923 18
ATCAG 2768335 2.2121296 33.622856 12
GTGGT 4014360 2.1884305 32.930954 15
TGCTT 4430245 2.188084 43.363647 19
CAGAC 2247305 2.1763058 35.752014 14
CTCTT 4204665 2.1760085 25.951326 4
AGTTC 3413320 2.1443272 13.827407 4
TGTTT 5229345 2.1311672 25.004488 4
TATCT 3860500 2.096933 7.541773 27
GGCAT 2667540 1.9381982 12.2339115 21
CGCAT 2543600 1.9365506 14.426282 3
TAGAA 2295220 1.9249882 32.756817 19
ACAAC 1776245 1.8917578 21.847078 22
AGAGT 2460905 1.8766958 16.88875 2
AGTGG 2642365 1.8322597 42.883034 14
ACGAC 1850940 1.7924632 44.219032 2
CAGAG 1903775 1.759465 19.434448 1
TGGCA 2365005 1.7183803 16.775532 4
CAGAT 2100445 1.67843 35.04232 9
CGACC 1794210 1.6554625 41.98072 3
AGAGA 1682485 1.6320297 10.376402 16
GAGTT 2702255 1.6201211 13.128216 3
CCGCA 1749320 1.614044 17.414223 2
CGGCT 2287050 1.5832554 18.583027 23
ACCTC 1969680 1.5713346 36.413975 5
GAATC 1964810 1.5700464 6.0650225 21
CTCAG 2056065 1.5653696 34.715736 7
GAATT 2372115 1.5640898 25.50398 21
AGACG 1681160 1.5537248 33.709038 15
GGTAG 2207735 1.5308801 27.3551 17
GGCTC 2161795 1.4965453 16.474373 24
GACCT 1963840 1.4951547 34.920372 4
GACAA 1462125 1.4861213 22.603638 21
CCTCA 1855040 1.4798791 36.11771 6
GAGAC 1594265 1.4734166 21.654894 19
GTTGG 2651190 1.4452977 12.882549 2
ACCGC 1527860 1.4097096 17.494587 1
AGACA 1386230 1.4089808 24.223032 20
TACGA 1761795 1.4078206 36.763256 1
GAAAG 1409050 1.3667946 14.724567 14
TTGGC 2389000 1.3646631 13.159314 3
CGGAT 1871305 1.3596646 7.571787 23
CGGCG 1696955 1.3586849 18.367067 10
TGGTT 2867570 1.3516285 16.877125 8
TTCAG 2147755 1.3492699 24.279877 11
GTTCA 2120990 1.3324556 24.449429 10
GACTG 1830925 1.330325 15.92646 11
CTGGA 1804330 1.3110014 16.280785 18
GAACT 1622605 1.2965963 8.843255 7
GAAGA 1327445 1.2876368 10.5684 14
CCATC 1603735 1.2793978 7.5432987 26
AGCTT 2033450 1.2774608 14.569846 12
AGGAT 1665950 1.27046 5.9104123 21
GCTCG 1827125 1.2648633 14.989125 25
AATTC 1826465 1.2619152 26.313793 22
GAGAG 1407760 1.2416544 9.612485 17
CGAGA 1338390 1.2369374 26.708822 18
CAGTG 1680260 1.2208538 28.536121 13
GGTTG 2193975 1.1960466 12.570518 1
TGGTA 1969575 1.1808472 23.516525 16
ACGAG 1268185 1.1720539 26.498709 17
TCAGT 1827370 1.1479965 25.391182 12
AAGAA 1070620 1.1421337 11.197338 5
TGAAT 1730150 1.1408005 5.67891 31
GCAGC 1286655 1.1329619 19.927687 6
CTGAA 1417670 1.132836 6.852178 30
ATTGA 1715835 1.1313616 10.101932 11
TGATC 1793275 1.1265773 6.465937 28
TTATC 2066085 1.1222488 11.497968 5
GCGGC 1401370 1.1220217 17.954601 9
GATCG 1540170 1.1190662 9.946265 16
CATTG 1777285 1.116532 27.524647 2
TGGAC 1529975 1.1116588 15.466066 19
GGCAG 1311470 1.102094 19.222912 5
AGACT 1372025 1.096362 17.071592 10
CTCGC 1504180 1.0911087 21.47917 26
AAGGA 1118690 1.085142 5.5039625 22
GGGAT 1562485 1.083453 11.089226 27
GGACG 1278995 1.0748037 17.412714 20
GGCGA 1268485 1.0659716 18.80351 11
GCATT 1696000 1.0654669 27.052706 1
ATTGG 1765955 1.0587679 23.536171 3
AAAGA 991275 1.0574888 11.127518 4
TGAAC 1320670 1.055325 5.09409 11
CGGCA 1186665 1.0449158 9.061565 23
CGTGG 1577080 1.0419242 15.755402 7
TCAGC 1359085 1.0347292 11.115672 19
GGAGC 1231040 1.0345047 19.675295 10
AAAAG 966655 1.0312241 11.166004 3
AAAGT 1220900 1.023962 12.512405 15
ATTCT 1883270 1.022948 20.643156 23
TCTGG 1790365 1.0227063 13.165491 17
GATCC 1339715 1.0199819 10.895259 28
GAAGG 1153595 1.0174791 5.4779706 17
ACTGG 1399105 1.0165704 15.646703 12
GGAAG 1146930 1.0116005 5.8529696 20
CAGAA 992505 1.008794 5.7841973 14
CTATC 1527790 1.0057052 7.8503113 31
CAGCG 1138995 1.00294 19.820688 7
TCGGA 1375345 0.9993068 7.49683 22
GGTTC 1732805 0.9898263 20.294537 9
AAGAG 1014570 0.9841445 10.194088 15
GAGCT 1338250 0.97235405 17.089703 11
TTCTC 1867020 0.96622473 19.297426 24
GTCAG 1319420 0.95867246 11.356076 18
GGATA 1239760 0.94544584 11.282572 28
GACTC 1232645 0.93846506 16.740995 14
TCGCC 1283645 0.9311361 18.896767 27
CGCCT 1266620 0.9187864 15.64368 28
AAGTC 1144990 0.9149421 11.835521 16
GCGAC 1037395 0.9134764 19.264069 12
CCAAG 942100 0.9123363 13.814361 31
CTCTG 1517430 0.90826154 13.37067 16
CAGCC 982530 0.9065502 13.380315 20
GCGGA 1075190 0.903536 5.1972795 18
TCGTG 1579055 0.90200007 6.772181 5
TATCA 1299045 0.8975176 14.677495 6
GCGCC 1066075 0.89439446 19.95793 3
ATCGT 1415375 0.88917166 8.355265 17
TTGGT 1884575 0.8882941 18.62195 4
TGAAG 1162760 0.8867253 8.245004 13
CAAGC 908010 0.8793233 13.709538 32
ATCCA 1041850 0.87234855 11.989894 29
AGCGC 988045 0.87002134 20.750546 2
TGAGA 1137645 0.86757255 6.965955 1
AGAAA 805435 0.8592353 7.083163 4
CAACC 841775 0.85417503 18.84114 23
AGCCC 924005 0.85255104 13.355251 21
TCCAA 1012570 0.84783214 12.010493 30
CTCGA 1109770 0.84491503 11.684222 25
TGCAT 1338520 0.8408896 6.181085 32
TAGGA 1092750 0.8333354 5.2254505 20
CGACT 1091100 0.83070076 17.013836 13
AGTCA 1031890 0.82456577 11.617751 17
AGTAG 1081095 0.8244473 7.4027762 7
CGCCG 979840 0.82204664 19.6845 4
GCTTA 1306185 0.8205759 13.285492 3
TACAA 929670 0.81700647 12.682874 2
GTGAA 1058950 0.80755943 5.1907234 10
GATAA 959735 0.80492437 5.585847 15
AAAAC 718565 0.80323124 5.0796695 32
GATTG 1328205 0.79631746 5.5107255 4
TCTGA 1266425 0.7955978 5.247102 1
TTTGA 1532675 0.79450774 5.7205553 11
GATAG 1040745 0.7936762 11.214511 29
CGGGA 940280 0.7901644 12.667194 26
CCTCG 1086935 0.78844583 10.5987215 24
TGGCC 1132355 0.78389513 10.586683 21
GTGGC 1182640 0.78133094 8.786507 7
CTGCG 1126535 0.77986604 10.370342 2
GCGAT 1069430 0.77703315 10.766052 4
TGTTG 1644235 0.7750099 9.832985 17
TGAGG 1117015 0.7745568 7.0364122 1
AACCC 760255 0.77145416 15.552095 24
GCCCT 1060610 0.76935 10.529369 22
ACAGA 755255 0.76765025 6.339311 19
CCCTC 1008680 0.7666808 11.078565 23
CACCG 821470 0.7579452 11.172774 6
AACAG 741390 0.7535577 6.4730487 18
GCGCA 854555 0.75247693 10.797327 3
CCGGG 928815 0.7436656 11.975514 25
GCCTG 1072990 0.74279845 7.223041 29
TCGAT 1181210 0.7420637 9.559351 26
CGTGA 1019515 0.7407656 5.9566426 28
CCCGG 868755 0.72885084 12.572363 24
TTCGG 1260015 0.71975565 10.574847 15
TAAGG 939320 0.7163291 11.813186 20
AAGGC 771170 0.7127138 11.002603 21
TAGCT 1130665 0.7103101 13.442884 1
AGGCA 768515 0.71026003 7.406541 22
GGTTA 1177310 0.7058493 13.171289 17
ATAAC 800455 0.7034506 5.6918793 16
CCGTG 1011435 0.7001858 16.191841 6
ATGAT 1060745 0.69941825 6.182285 27
GGCCC 832895 0.69876575 12.707423 22
TGCGA 958640 0.69653475 10.847751 3
CCTGC 956610 0.69391 7.2595997 30
GTACA 863245 0.6898045 12.198248 1
GCTGC 988730 0.6844678 10.224594 1
CTTAT 1249895 0.67891365 11.501331 4
CTGCA 890375 0.6778803 7.4352818 31
GCACC 726470 0.67029154 11.183441 5
TAGTG 1116650 0.6694809 6.724994 13
ATCTA 947695 0.654768 10.115591 7
GCTTC 1074810 0.64333016 13.788431 13
AACTT 928690 0.6416373 7.4423676 8
ATAGC 800440 0.63961804 11.574728 30
CTTCG 1068465 0.6395324 11.037591 14
ACCAT 749085 0.62721425 5.738621 25
GCCGA 710530 0.62565595 10.930092 10
CTTTG 1252155 0.6184354 5.3753047 10
GAAAT 732980 0.6147462 5.786502 5
TCGGT 1072180 0.612459 10.587994 16
GCTGA 833485 0.60559875 6.2042575 29
CGCAC 644930 0.59505713 11.213117 4
CCGAA 614345 0.5949361 5.651243 15
GTAGT 984540 0.5902752 5.6403136 5
CGGAA 632440 0.5844998 5.1111903 19
TAGTA 882010 0.5815666 5.9367166 6
AGCCT 762905 0.58083194 10.956168 32
TATTG 1102815 0.57167697 7.7095284 10
GGTGT 1033430 0.56337494 11.261134 15
CGAAG 604870 0.5590196 5.506598 16
GTTGT 1175475 0.55406 5.656884 1
GCCCG 659265 0.553097 12.415689 23
CCTAT 830255 0.5465356 7.378662 30
GAGGT 782080 0.54230726 6.7128105 2
ACCCG 578585 0.5338426 11.168266 25
GGCCG 664400 0.5319589 9.929132 9
TCTAT 978970 0.53175354 7.9700394 8
TTAAG 802565 0.52918327 11.2133875 19
AGGTA 689980 0.52618146 7.056341 3
CCCGC 593980 0.522163 10.280972 26
TAGCC 680800 0.51832193 11.045037 31
ACTTT 951445 0.5168026 5.7707963 9
CGCTG 727790 0.503827 5.883678 28
CGGTT 832925 0.4757899 10.25583 17
TAACA 541305 0.4757061 5.392049 17
ATGCG 647390 0.47038463 8.929893 1
CCCTG 646905 0.46925485 7.1335874 2
CCGAG 528310 0.46520248 10.918223 11
CTATT 853955 0.46384832 7.9640694 9
GAGTG 667730 0.46301505 8.041838 13
GTGTT 974475 0.4593186 9.708087 16
GTTAA 670055 0.44181088 8.205067 18
GTCGT 762360 0.43548125 6.8153477 4
TGTCG 728470 0.41612238 6.898245 3
TGCGC 583060 0.40363482 8.432128 2
GCCTA 526145 0.40057653 8.436292 29
CCCGA 431920 0.39851934 5.183274 14
CGAGT 544900 0.39591685 8.175358 12
TCCGT 660120 0.39511645 6.9269695 3
CCGCT 521445 0.37824813 7.7380514 27
TCCCT 595645 0.3735792 6.077634 1
TTGTC 724550 0.3578529 6.0680437 2
Produced by FastQC (version 0.10.0)