FastQCFastQC Report
mer. 23 nov. 2011
s_2_uT22.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename s_2_uT22.fastq
File type Conventional base calls
Encoding Illumina 1.5
Total Sequences 91418250
Filtered Sequences 0
Sequence length 36
%GC 43

[FAIL] Per base sequence quality

Per base quality graph

[FAIL] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA 18683288 20.437153413022017 Illumina Single End Adapter 2 (95% over 24bp)
TAGCTTATCAGACTGGTGTTGGCATCTCGTATGCCG 4826776 5.279882299212685 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG 1248852 1.366086093312878 Illumina Single End Adapter 2 (95% over 24bp)
TGAGAACTGAATTCCATAGATGGATCTCGTATGCCG 1066526 1.1666445157285332 No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGCCGTCT 823460 0.9007610624793191 RNA PCR Primer, Index 38 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCATCTCGTA 738498 0.8078233831866175 No Hit
TAGCTTATCAGACTGGTGTTGGATCTCGTATGCCGT 683306 0.7474503176335141 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC 647362 0.708132128978623 Illumina Single End Adapter 2 (95% over 24bp)
TGAGGTAGTAGGTTGTATAGTTATCTCGTATGCCGT 633237 0.6926811659597509 No Hit
TTCCTATGCATATACCTCTTTATCTCGTATGCCGTC 515911 0.5643413650994195 No Hit
TGAGAACTGAATTCCATAGATGGTATCTCGTATGCC 495956 0.5425131196451475 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT 489401 0.5353427789309028 Illumina Single End Adapter 2 (95% over 24bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 442494 0.4840324552263908 No Hit
GCATGTTTGTGGAGAACCTGGTGCTAAATCACTCGT 371779 0.406679191518105 No Hit
TGAGGTAGTAGATTGAATAGTTATCTCGTATGCCGT 363491 0.3976131680490493 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA 350568 0.3834770409628274 Illumina Single End Adapter 2 (95% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA 337614 0.3693070037984757 Illumina Single End Adapter 2 (95% over 24bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTATGCCGT 332394 0.3635969841907934 No Hit
ATGTTTGTGGAGAACCTGGTGCTAAATCACTCGTAT 237819 0.2601438990573545 No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTATGCCG 227534 0.24889341023263956 No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA 222564 0.2434568589969727 Illumina Single End Adapter 2 (95% over 23bp)
ATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA 201640 0.2205686501327689 Illumina Single End Adapter 2 (100% over 21bp)
TGTAAACATCCCCGACTGGAAGCATCTCGTATGCCG 200728 0.21957103751165657 No Hit
TGTAAACATCCTTGACTGAAAGCATCTCGTATGCCG 193159 0.21129150908051728 No Hit
TACCCTGTAGAACCGAATTTGTATCTCGTATGCCGT 186726 0.20425462093181615 No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTATCTCGTAT 182574 0.19971285820938378 No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAT 177014 0.19363092161576054 No Hit
GTGGAGAACCTGGTGCTAAATCACTCGTATGCCGTC 174076 0.19041712130783514 No Hit
TTTCCTATGCATATACCTCTTTATCTCGTATGCCGT 173209 0.1894687329936856 No Hit
AAGCTGCCAGCTGAAGAACTGTATCTCGTATGCCGT 162674 0.17794477579695522 No Hit
ATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA 159721 0.1747145673867089 Illumina Single End Adapter 2 (95% over 22bp)
CAACTCTTAGCGGTGGATCACTCGGCATCTCGTATG 151088 0.1652711575642719 No Hit
TTCCTATGCATATACCTCTTTGAATCTCGTATGCCG 145721 0.15940033855384456 No Hit
TGAGGTAGTTGGTTGTATGGTTATCTCGTATGCCGT 139003 0.1520516964610458 No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTC 136474 0.14928529040973768 No Hit
TACCCTGTAGATCCGGATTTGTGATCTCGTATGCCG 134865 0.14752524796744632 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA 132671 0.14512528953463888 Illumina Single End Adapter 2 (95% over 24bp)
TACCCTGTAGATCCGGATTTGTATCTCGTATGCCGT 125695 0.13749442808192017 No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCT 124067 0.1357136020433557 No Hit
TACAGTACTGTGATAACTGAAGATCTCGTATGCCGT 121593 0.13300735903389094 No Hit
ATAGCTCTTTGAATGGTACTGCATCTCGTATGCCGT 121420 0.13281811892045625 No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGTATGCCG 117733 0.12878500736997264 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG 113160 0.1237827239090663 Illumina Single End Adapter 2 (95% over 24bp)
TCCCTGAGACCCTAACTTGTGAATCTCGTATGCCGT 111655 0.12213644430953338 No Hit
TGAGATGAAGCACTGTAGCTCGGATCTCGTATGCCG 107429 0.11751373494898448 No Hit
TGTGGAGAACCTGGTGCTAAATCACTCGTATGCCGT 101241 0.11074484580485844 No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTATGCCGT 94974 0.10388954065517553 No Hit
ATGCGCACCGCATGTTTGTGGAGAACCTGGTGCTAA 94958 0.10387203867936654 No Hit
TGTAAACATCCTACACTCTCAGCATCTCGTATGCCG 93377 0.10214262469474093 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 211873780 45.484974 183.9745 25
TGCCG 53164360 29.122454 434.95868 9
TCTCG 69928175 28.93658 328.75354 2
CTCGT 69089395 28.589487 327.40634 3
ATGCC 55935315 24.456429 346.95898 8
GCCGT 42161995 23.09556 430.09125 10
ATCTC 69242520 22.870073 262.88614 1
TCGTA 68466495 21.617685 249.63495 4
CGTAT 64603425 20.397957 249.42311 5
CCGTC 34575830 19.812693 446.429 11
GTATG 61884905 18.678942 239.81544 6
TATGC 58748545 18.549328 249.46953 7
CGTCT 32551805 13.4700775 324.15942 12
TCTGC 29582780 12.241482 325.9925 17
CTGCT 29146500 12.060946 324.48813 18
GCTTG 29177655 11.542021 312.11343 20
CTTCT 30454535 9.519949 246.83131 15
TCTTC 30220390 9.446756 244.39644 14
CTTGA 29725020 9.38541 248.51004 21
GTCTT 30692150 9.171629 235.18315 13
TTCTG 30604145 9.145329 237.51414 16
TGCTT 29415640 8.790174 235.76982 19
GAAAA 30424350 8.182998 215.12874 24
TGAAA 30810005 7.8427954 202.79585 23
TTGAA 31007780 7.470297 191.39131 22
CATCT 19036795 6.287651 74.7034 23
GCATC 12726450 5.5643477 95.669464 22
TGGTG 12417860 4.695855 83.71033 14
CTGGT 10437360 4.1287837 86.8345 13
GACTG 9770020 4.0835595 93.879845 11
GGCAT 9119855 3.8118107 78.86247 21
CAGAC 8129325 3.755549 101.258415 9
ACTGG 8945370 3.7388818 93.832855 12
GTTGG 8694105 3.2877047 81.849464 18
GATCT 10387190 3.2796628 20.89356 23
TAGCT 10352215 3.2686198 71.01328 1
TGGCA 7501895 3.1355548 77.29888 20
TCAGA 8991800 2.999782 77.775986 8
GGTGT 7887480 2.9826767 81.90778 15
TATCT 12009165 2.8643858 29.500404 22
TTATC 11799220 2.8143108 53.49873 5
GGATC 6649075 2.779103 20.080975 22
AGACT 8245300 2.75074 74.03972 10
ATCAG 8222515 2.7431386 74.24159 7
TTGGC 6682750 2.643545 73.59967 19
AGAAC 7388980 2.604591 24.133842 3
GTGGT 6691670 2.5304773 32.839207 15
GCTTA 7907750 2.4968016 70.797935 3
GGTGG 4944200 2.475006 36.716038 6
AGCTT 7792030 2.460264 70.94616 2
TGTTG 8281930 2.3658495 60.94535 17
GGTAG 5665080 2.2635283 28.141087 17
GTGTT 7793660 2.2263684 60.606453 16
GAGAA 5946365 2.0037477 22.750805 2
CTTAT 8390025 2.001161 53.533928 4
TATCA 7841445 1.9761814 56.063396 6
GTAGA 6149120 1.9610672 22.460672 18
TGAGA 6063300 1.9336977 42.74986 1
CCTGG 3254720 1.7828755 9.10794 17
AATCT 6931780 1.74693 6.900384 22
CTGAA 5195705 1.7333552 21.175228 7
GTGGA 4327885 1.7292414 8.884996 13
AGTGG 4316215 1.7245786 34.97893 14
GAAGC 3820240 1.6871204 30.380623 7
CAGTG 3899800 1.6299932 28.355988 13
GAACC 3524875 1.6284058 8.698848 10
AGATG 5074040 1.6182047 21.300575 3
GGAGA 3821840 1.6134828 6.7581716 11
ACTGA 4821965 1.6086706 21.552034 6
TGTAG 5329010 1.6084741 20.45767 14
CACTC 3497755 1.5997787 9.178919 19
TGGAT 5284465 1.595029 12.473927 21
ACTCG 3637760 1.5905268 8.895717 20
CTGTA 4941460 1.560222 21.489029 13
GAATT 6371690 1.5350478 16.10829 21
AAGCA 4336930 1.5287535 23.919006 8
TGAAG 4701285 1.4993261 21.854595 6
TGGTT 5233470 1.4950142 18.829115 8
GAACT 4467255 1.4903347 22.05235 4
GATGA 4566000 1.4561814 22.43505 4
TGGAG 3632620 1.4514425 6.101409 10
CACTG 3294145 1.440289 30.314342 11
CTCGG 2611415 1.4304848 10.49173 21
GGTTC 3575220 1.4142764 26.138649 9
TGGTA 4676085 1.4113996 20.823143 16
AACTG 4217625 1.4070548 21.187584 5
AGCAC 3020435 1.3953669 30.891474 9
TGAGG 3483920 1.3920282 27.089521 1
TGTGG 3670155 1.3878813 6.220727 8
ACTGT 4389035 1.3857987 22.118832 12
TGAAT 5646825 1.3604153 15.305051 8
GCACT 3086350 1.3494356 29.83928 10
GGTGC 2524680 1.3220569 7.941355 20
GATGG 3308455 1.3219199 24.46532 19
CTATC 3992180 1.3185749 10.630307 19
ACCTG 2980235 1.3030392 8.780358 16
TAGAA 5072995 1.2913488 17.27299 19
ATCAC 3617235 1.2623603 6.834846 17
GTAGC 2998170 1.2531402 27.053118 15
AATTC 4937785 1.2444084 16.22911 22
CCCTG 2158620 1.2369356 14.5686655 3
AAAAG 4574735 1.2304304 14.948413 32
CTCTT 3881740 1.2134143 9.509876 16
AGCAT 3592665 1.19856 10.8769 21
GTATC 3770550 1.1905174 8.483774 21
GAGGT 2969540 1.1865036 24.26504 2
GGAAG 2799545 1.1818961 10.052793 18
ATGAA 4631395 1.1789379 17.12176 5
TCATC 3558895 1.1754655 12.231256 27
GAATC 3498530 1.1671555 5.0382795 22
ACATC 3342705 1.1665534 14.64853 6
AGAAT 4553905 1.1592127 17.490072 20
TCAGT 3669860 1.1587256 24.234879 12
GTGCT 2903870 1.1487054 5.8493004 21
AGCGG 2058690 1.1390603 11.916424 9
CATTG 3599575 1.1365337 24.008745 2
GGTTG 3004820 1.1362827 16.761532 11
GAGAT 3554400 1.1335634 22.073679 2
CTGGA 2663105 1.1130936 9.903884 16
AGATC 3298600 1.1004562 5.0345893 21
GTAGG 2751100 1.0992241 14.688561 8
TTGGT 3813425 1.0893584 19.556427 4
AGCTC 2487450 1.0875803 14.387422 17
TCACT 3261395 1.0772041 6.5989223 18
CCGGC 1409075 1.0688515 8.021632 11
TTCAG 3343770 1.0557656 20.569574 11
TGCAT 3317960 1.0476164 10.130285 7
TCGGC 1911705 1.0471966 9.14236 22
AAACA 3713025 1.0446782 11.682692 4
TTCTC 3332810 1.0418212 17.333704 24
CGGTG 1987120 1.0405618 10.894863 11
AAAAC 3693185 1.039096 7.68763 32
GCATT 3247100 1.0252429 23.567944 1
GTTCA 3246320 1.0249966 20.664406 10
CGGCT 1850040 1.0134176 5.794045 12
TAGAT 4197045 1.0111389 14.868728 17
TGGGG 2019020 1.0106968 5.6137686 14
TGGAA 3133840 0.9994391 7.514404 17
ACCTC 2178745 0.9964991 13.182308 14
GTAGT 3269160 0.98674226 18.190996 5
ATTGG 3250230 0.98102856 21.032364 3
TCCAT 2904180 0.9592198 21.058271 13
GTCGG 1812720 0.94923675 6.7411394 14
GCGGT 1798475 0.9417773 12.178494 10
AACCT 2695705 0.94076014 6.2255955 15
GTGGG 1873500 0.93785125 5.0084767 18
CCTCT 2160070 0.93503183 13.444003 15
ATGCA 2790070 0.93080384 10.451269 6
TTGGA 3028640 0.9141453 9.155383 19
ATTCC 2760550 0.91178036 20.607525 11
GGGGT 1815890 0.9090124 6.2174444 15
GGGCA 1640445 0.9076479 5.016383 17
CCTGT 2188645 0.90567076 10.523105 4
AGTAG 2835580 0.90431863 17.649046 7
AAATC 3385655 0.90154076 5.246543 26
GATCA 2672975 0.8917394 6.367653 16
TGTAT 3873250 0.8831425 10.934547 14
AGGTA 2752325 0.87776697 18.958878 3
AACAT 3291645 0.8765075 11.169159 5
CCTAT 2651180 0.8756568 10.462373 3
ATGGA 2738230 0.8732718 13.117923 20
AACTC 2498040 0.8717781 7.3394933 2
ACTCT 2638560 0.87148833 7.155 3
TTCCA 2637695 0.87120265 20.899742 12
GGCCG 1198045 0.868746 10.374494 9
GCTAA 2600645 0.8676092 5.2625365 23
CCATA 2457330 0.8575709 21.368065 14
GCCGG 1152865 0.83598435 8.195996 10
CATAG 2475750 0.8259426 20.601702 15
CTCAT 2494865 0.8240274 11.510118 26
CTGGG 1568420 0.8213082 5.1486816 13
CGGCA 1417630 0.8205068 5.138934 23
CATCC 1792350 0.8197725 19.00553 7
TTTGT 3788000 0.81743497 5.7523947 18
CTCAG 1857070 0.8119611 6.222414 7
GTTGT 2837695 0.8106274 12.583424 12
AAAGA 2978970 0.8012301 5.129333 32
TCTCA 2413380 0.7971138 11.357652 25
GCATG 1906090 0.7966854 6.994048 1
TTGTA 3484665 0.7945409 10.697486 13
ATTCT 3309550 0.7893828 14.794092 23
ACCCT 1677880 0.76741695 11.933029 2
TAGTA 3174035 0.7646785 12.4065275 6
CTATG 2412415 0.76169854 10.071534 4
TGATG 2494850 0.75302947 5.0807652 7
GCATA 2252910 0.75160027 10.227113 8
GCTCT 1797150 0.74366844 8.755847 18
TCCTA 2247490 0.7423221 10.281425 2
ATAGA 2866505 0.72967887 15.595601 16
TAGCG 1740525 0.7274844 8.936808 8
CTCGC 1248565 0.71545446 11.779587 26
ATACC 2034035 0.70984733 10.600174 12
TGACT 2231090 0.7044468 6.864544 13
GCTAT 2213150 0.6987824 10.053887 18
GCCGA 1204635 0.6972279 7.7619314 10
ATGGT 2283605 0.68926865 5.910172 20
CGCCT 1201730 0.6886171 7.6975894 28
TGTAA 2836225 0.6832944 10.3201275 1
GATTG 2253580 0.6802061 5.4567 11
TTCCT 2167820 0.6776507 10.094077 1
AGCTA 2021895 0.6745306 10.507415 17
GCTGT 1704275 0.6741727 5.426802 11
CTGCC 1170595 0.670776 5.347458 4
TTGAC 2118110 0.66877437 6.777485 12
GGTCG 1275615 0.6679799 5.4781756 4
CTGTC 1613335 0.667605 5.410573 12
GTGGC 1263270 0.6615155 6.955556 7
CATAT 2605135 0.6565397 7.787893 9
AGGGC 1185900 0.6561511 5.0109215 16
GGTAT 2125905 0.6416695 6.081341 22
AGGTT 2106780 0.6358969 10.738751 10
GTTAT 2784455 0.63488555 13.086024 20
GCTCA 1432420 0.6262927 6.6216245 10
GACGA 1416775 0.62568575 5.8486786 16
AAAAT 3048220 0.6193342 5.110267 32
GGTTA 2035085 0.61425704 6.373385 17
GACCT 1400545 0.6123561 5.8876805 4
TTAGC 1912185 0.6037554 7.519338 7
CAACT 1728390 0.6031819 7.3632736 1
CCCCG 759415 0.60259557 8.926954 10
CCGGA 1034375 0.59868354 6.6375127 13
CCGAA 1289280 0.59561574 6.546056 13
TCTTT 2625990 0.5927886 7.1614394 17
GCTGC 1081560 0.5924586 5.50778 3
AGACG 1325160 0.5852262 5.508287 15
TCGCC 1015150 0.5817027 10.799823 27
CTTAG 1825420 0.5763601 6.87648 6
CATGT 1825040 0.5762401 5.199512 2
GTAAA 2245950 0.57171446 10.311877 2
ATTGA 2371355 0.5712993 5.870613 12
TACCT 1715635 0.566656 9.262163 13
TCCCT 1308025 0.56620616 7.016861 1
TAGTT 2482455 0.5660263 10.409795 18
CCGAC 932155 0.56437916 6.6526194 12
TAGGT 1850835 0.5586441 10.304472 9
ATCCT 1690080 0.55821544 10.065506 8
CCTCA 1213985 0.5552439 5.9775286 6
AACCG 1195825 0.5524418 6.6428246 11
ATAGT 2284165 0.55029374 11.347147 17
TCTTA 2298355 0.54819596 5.105699 5
GCCAG 934150 0.5406745 5.512122 6
TCCGG 980550 0.53712714 6.3197575 12
CCTTG 1290320 0.53394 8.736389 10
ATCCC 1167075 0.53378856 6.6512666 8
TACCC 1117165 0.5109611 11.441719 1
GATCC 1156505 0.5056552 5.7982984 10
TAAAC 1890200 0.5033272 10.672844 3
CGCTG 915765 0.5016391 5.0078883 10
CCAGC 826385 0.50034004 5.5954566 7
TCCCC 820390 0.49176174 7.0307446 9
GTATA 2039225 0.49128363 7.8957167 15
TATAG 1947120 0.46909398 8.026111 16
TATAC 1843305 0.4645451 7.7462835 11
CTTTA 1936945 0.46199366 5.568368 18
CGACC 751920 0.45525473 8.024692 3
TGGCC 827115 0.45307827 5.941936 8
TCCTT 1443425 0.45120808 6.694616 9
AGTTA 1817920 0.43796757 10.655343 19
ACGAC 946290 0.4371628 6.2496676 2
CCCGA 721115 0.43660367 6.742968 11
ACCGA 944750 0.43645135 6.5840654 12
ATTTG 1855380 0.42304644 5.9763813 17
ATATA 1942665 0.3735637 5.934087 10
CCGAG 592720 0.34305903 5.758648 11
Produced by FastQC (version 0.10.0)