FastQCFastQC Report
mer. 23 nov. 2011
s_2_uT5.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename s_2_uT5.fastq
File type Conventional base calls
Encoding Illumina 1.5
Total Sequences 96110022
Filtered Sequences 0
Sequence length 36
%GC 42

[FAIL] Per base sequence quality

Per base quality graph

[FAIL] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA 21056498 21.9087432942217 Illumina Single End Adapter 2 (95% over 24bp)
TAGCTTATCAGACTGGTGTTGGCATCTCGTATGCCG 5266047 5.479186135239882 No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGCCGTCT 3598552 3.7442005787908363 RNA PCR Primer, Index 38 (100% over 21bp)
TGAGATGAAGCACTGTAGCTCTATCTCGTATGCCGT 2860045 2.9758030853431707 No Hit
TGAGAACTGAATTCCATAGATGGATCTCGTATGCCG 1621463 1.687090447237646 No Hit
TTCCTATGCATATACCTCTTTATCTCGTATGCCGTC 938379 0.9763591563843363 No Hit
TAGCTTATCAGACTGGTGTTGGATCTCGTATGCCGT 909653 0.9464704939928117 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG 879673 0.9152770769316856 Illumina Single End Adapter 2 (95% over 24bp)
TGAGGTAGTAGATTGAATAGTTATCTCGTATGCCGT 721646 0.7508540576548822 No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTATGCCGT 550729 0.5730193257057 No Hit
TGAGAACTGAATTCCATAGATGGTATCTCGTATGCC 536211 0.5579137210061195 No Hit
TACAGTACTGTGATAACTGAAGATCTCGTATGCCGT 455941 0.4743948555125708 No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 412772 0.42947862398782927 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC 412105 0.42878462768430126 Illumina Single End Adapter 2 (95% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT 357160 0.37161577176623684 Illumina Single End Adapter 2 (95% over 24bp)
TCAGTGCATAACAGAACTTTGAATCTCGTATGCCGT 327017 0.3402527574075469 No Hit
TGAGATGAAGCACTGTAGCTCTCTCGTATGCCGTCT 325243 0.3384069561444903 RNA PCR Primer, Index 38 (95% over 21bp)
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA 290654 0.30241799341175885 Illumina Single End Adapter 2 (95% over 23bp)
TTTCCTATGCATATACCTCTTTATCTCGTATGCCGT 274838 0.2859618531769767 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA 274561 0.28567364181854 Illumina Single End Adapter 2 (95% over 24bp)
CCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA 274533 0.2856445085404309 Illumina Paired End PCR Primer 2 (96% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAT 271368 0.2823514076398817 No Hit
TGTAAACATCCTTGACTGAAAGCATCTCGTATGCCG 258991 0.2694734582414309 No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA 239783 0.24948802945857196 Illumina Single End Adapter 2 (95% over 24bp)
TGAGATGAAGCACTGTAGCTCTTATCTCGTATGCCG 216578 0.22534382522563567 No Hit
TTCCTATGCATATACCTCTTTGAATCTCGTATGCCG 209627 0.2181114889350457 No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTATGCCG 202981 0.21119649728100157 No Hit
AAGCTGCCAGCTGAAGAACTGTATCTCGTATGCCGT 195522 0.20343560008757464 No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTC 186973 0.19454058599632826 No Hit
TGAGATGAAGCACTGTAGCTCTATCGTATGCCGTCT 167312 0.17408382239263248 No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTATGCCGTC 166366 0.17309953378223136 RNA PCR Primer, Index 10 (95% over 21bp)
TGAGATGAAGCACTGTAGCTCGATCTCGTATGCCGT 165615 0.17231813764437595 No Hit
ATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA 160016 0.16649252249676938 Illumina Single End Adapter 2 (100% over 21bp)
TGTAAACATCCTACACTCTCAGCATCTCGTATGCCG 159195 0.16563829316364115 No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTATGCCG 153615 0.15983244702618005 No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATGCCGTC 153179 0.15937880026705228 RNA PCR Primer, Index 44 (95% over 21bp)
TTCCTATGCATATACCTCTTTGATCTCGTATGCCGT 149859 0.15592442586268473 No Hit
ATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAAA 138081 0.14366972052092547 Illumina Paired End PCR Primer 2 (95% over 24bp)
TCAGTGCATAACAGAACTTTGATCTCGTATGCCGTC 128962 0.1341816361253148 No Hit
TGAGAACTGAATTCCATAGATGATCTCGTATGCCGT 127745 0.1329153790017861 No Hit
GCATGTTTGTGGAGAACCTGGTGCTAAATCACTCGT 124378 0.12941210230916397 No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATGCCGTCT 122177 0.1271220185549432 RNA PCR Primer, Index 38 (95% over 21bp)
TCAGTGCATTACAGAACTTTGAATCTCGTATGCCGT 120248 0.12511494378806823 No Hit
TGAGATGAAGCACTGTAGCTCGGATCTCGTATGCCG 113773 0.11837787322533336 No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGTATGCCG 112243 0.11678594767151337 No Hit
ATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA 107650 0.11200704958739892 Illumina Single End Adapter 2 (95% over 22bp)
TGAGATGAAGCACTGTAGCATCTCGTATGCCGTCTT 104832 0.10907499324055925 RNA PCR Primer, Index 24 (96% over 26bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 235285015 47.239494 191.27002 30
TGCCG 66671000 35.333755 463.19748 9
TCTCG 76792860 30.36917 346.93802 2
CTCGT 74838730 29.59637 345.01547 3
ATGCC 68681365 28.766565 366.42572 8
GCCGT 53857655 28.543043 456.35724 10
CCGTC 42406920 23.470459 469.87845 11
ATCTC 74348020 23.236876 270.1755 1
TCGTA 76000900 22.745476 261.90314 4
CGTAT 73787190 22.082958 261.8253 5
TATGC 71175085 21.301212 261.2074 7
GTATG 72033990 20.643423 252.68893 6
CGTCT 39775850 15.730102 338.3412 12
TCTGC 32445615 12.8312235 337.03683 17
CTGCT 31896915 12.61423 334.7213 18
GCTTG 32091230 12.152521 324.11096 20
CTTCT 33901235 10.004302 255.50067 15
TCTTC 33360420 9.844707 252.0672 14
CTTGA 32851185 9.831671 255.69041 21
GTCTT 33830125 9.559669 243.58304 13
TTCTG 33382780 9.43326 245.82158 16
TGCTT 32125625 9.078013 241.12119 19
GAAAA 34652035 8.803258 224.10635 24
TGAAA 34012215 8.158542 210.5744 23
TTGAA 35236180 7.980486 197.41599 22
TAGCT 19601240 5.86624 96.46578 16
CATCT 17759020 5.550439 78.13595 23
GAAGC 12993265 5.5191526 152.42351 7
AGCAC 11926465 5.2905145 154.1223 9
GCATC 12222685 5.119361 102.655136 22
CACTG 12104465 5.069845 147.7578 11
GCACT 11913885 4.990023 148.13855 10
TGAGA 15684165 4.760392 138.62613 1
GTAGC 11463765 4.597739 134.80066 15
AAGCA 13332280 4.475637 119.070984 8
AGATG 14727125 4.4699154 105.71794 3
GACTG 11030735 4.424065 110.21339 11
GGGGG 6661205 4.3379273 6.1579485 15
TGAAG 13835435 4.199273 107.24454 6
ACTGG 10340585 4.147269 111.021805 12
ACTGT 13558360 4.0577326 104.94152 12
CAGAC 9055105 4.0167947 116.457504 9
TATCT 17825710 3.98091 48.375523 22
TGGTG 10819245 3.923241 95.849655 14
CTGGT 10291605 3.8972936 97.98256 13
CTGTA 12917340 3.8658888 103.15316 13
TGTAG 13377545 3.8337224 99.757545 14
GATGA 12491535 3.7913785 106.56679 4
GAGAT 12132750 3.6824815 106.64849 2
GGCAT 8885015 3.5634873 85.933945 21
GTTGG 9370650 3.3979554 92.91671 18
GGTGT 9266950 3.360352 94.19365 15
CTATC 10124550 3.1643467 43.336067 19
TGGCA 7700690 3.0884938 83.68585 20
TTATC 13744475 3.069472 60.70301 5
ATGAA 12667325 3.038523 83.69462 5
TCAGA 9557945 3.0295484 85.34074 8
AGACT 9251360 2.9323711 84.668365 10
AGCTC 6924710 2.900352 77.57841 17
ATCAG 9090000 2.8812256 84.99326 7
GATCT 9294950 2.7817836 27.342178 22
TTGGC 7286970 2.7594783 79.72473 19
AGCTT 9176955 2.7464702 81.79176 2
GCTTA 9117865 2.7287858 81.83493 3
TGTTG 9581915 2.5927434 68.110565 17
GTGTT 9187105 2.4859128 68.067345 16
GCTCT 5867935 2.3205843 63.24228 18
CTTAT 9986765 2.230285 60.910557 4
GGATC 5526690 2.2165737 28.334288 22
AGAAC 6444160 2.1633 33.370987 3
TATCA 8975815 2.1229827 63.386845 6
CTGAA 6065635 1.9226031 29.805826 7
GAACT 5655260 1.792528 30.68283 4
GGTAG 4601145 1.7670586 30.498693 4
ACTGA 5559205 1.7620817 30.585936 6
GAGAA 5180410 1.6652619 32.724087 2
GCTAT 5471945 1.6376383 41.930798 18
AACTG 5133150 1.6270366 30.415697 5
GGTGG 3340175 1.6231474 13.654556 6
AGCTA 5108565 1.619244 44.25648 17
GATGG 4024420 1.5455687 33.44558 19
TGAGG 3913445 1.5029491 35.119003 1
CCATC 3361490 1.4703238 6.9423227 6
TGCAT 4894310 1.4647644 17.37947 7
CTCTA 4656995 1.4555063 39.63751 19
TGAAT 6391620 1.4476098 21.387789 8
TGGAT 4859885 1.3927408 18.445509 21
GTGGT 3766765 1.3658925 12.733812 15
AATCT 5679980 1.3434433 9.352498 22
GTAGA 4391395 1.3328578 11.825408 8
CCTCT 3220960 1.3302382 24.333479 15
ATGCA 4176045 1.3236663 18.09404 6
GAGGT 3396415 1.304385 31.451967 2
GCCGG 1792350 1.2729648 13.530423 10
GTAGG 3269870 1.2557857 16.930162 8
CTCTT 4243940 1.2523926 17.06528 16
ACCTC 2828180 1.2370527 24.003311 14
TGGGG 2536305 1.2325094 7.5882993 14
TCCAT 3874115 1.2108235 29.859978 13
GGTTG 3287580 1.1921319 16.036715 11
TAGAT 5248965 1.1888149 20.59767 17
CCGGC 1591165 1.1801598 13.061581 11
CAGTG 2877035 1.1538842 12.231548 2
GGGGT 2364895 1.1492132 7.1979113 15
ATTCC 3676830 1.1491637 29.304077 11
GCATA 3565705 1.1302091 17.98848 8
CTCTC 2731195 1.1279681 7.3690195 21
AAAAG 4439125 1.1277479 12.70327 32
GTGGG 2300905 1.1181175 6.604196 18
CCATA 3376595 1.1176976 30.595171 14
TTCCA 3558950 1.1123213 29.93615 12
GTATC 3592750 1.0752348 6.768758 23
ACATC 3200440 1.059388 17.319944 6
GGATG 2756405 1.0585907 6.116604 6
CATAG 3336145 1.0574462 29.658672 15
AGTAG 3479585 1.0561091 24.150381 7
GAATT 4655830 1.0544783 21.34686 9
GTAGT 3673680 1.0527993 22.481276 5
GGAAG 2582175 1.0502864 9.045464 18
CTGGA 2612145 1.0476456 9.29803 16
TCTAT 4689825 1.0473508 27.425627 20
CCTAT 3292215 1.0289553 18.73602 3
AAACA 3876825 1.0285441 13.416958 4
GAATC 3228870 1.0234436 8.405982 21
TCCTA 3265855 1.0207168 18.172117 2
AATTC 4259335 1.0074288 22.062075 10
AGCAT 3137885 0.9946044 10.8165655 21
GAAGA 3079765 0.9900018 7.8508544 19
CGGCT 1849715 0.98029697 9.619265 12
TGGAG 2550830 0.97964007 5.8877687 14
ATGGA 3210690 0.9744952 19.288746 20
AGGTA 3133600 0.95109713 23.875797 3
TGTGG 2614045 0.9478967 5.5666094 17
ATACC 2857130 0.94574785 18.485508 12
TAGTA 4117870 0.9326381 16.667704 6
AGTGG 2421160 0.9298406 12.97239 14
TTCCT 3082830 0.9097475 17.807648 1
ATAGA 3777925 0.90621436 21.865257 16
TGGAA 2932305 0.89000094 6.847406 17
TGGTA 3053755 0.8751419 7.266026 16
CATCC 1999380 0.8745336 22.833103 7
AAAAC 3295360 0.87427807 6.666217 31
CTATG 2908815 0.8705473 17.55029 4
TGGTT 3201690 0.8663363 6.7165728 8
CCCCC 1069495 0.86510426 15.25822 1
TTGGA 3013805 0.86369294 12.375827 19
AGATC 2670070 0.8463228 9.057095 21
TTCTC 2778265 0.81986994 7.528942 24
TACCT 2600400 0.8127341 16.876175 13
AAAGA 3184105 0.80891347 5.3815985 32
CAGAA 2403640 0.8069002 10.741147 12
TCAGT 2632645 0.78789544 9.322454 1
GTTGT 2898275 0.784236 11.973561 12
CATAT 3256020 0.7701223 13.471665 9
TCTGG 2004450 0.7590585 5.8500395 17
CTCGC 1363645 0.75472057 12.687621 26
CACTC 1696920 0.74223685 6.360311 14
GATTG 2583005 0.74023473 10.997118 11
GATGC 1817530 0.7289515 7.1559343 7
TCTTT 3403705 0.71771234 12.599726 17
AACAT 2832030 0.70942664 13.093569 5
CTGTG 1866015 0.706635 9.935547 8
GGCTT 1855960 0.7028273 7.2482595 13
TAGAA 2927895 0.7023169 6.0665855 19
TGTAT 3237055 0.69223505 9.568455 14
GGCCG 972135 0.6904308 7.35504 9
TTGTA 3200630 0.6844457 9.40294 13
TGATC 2271585 0.67983776 5.1505036 21
ACAGA 1996500 0.6702236 10.338559 11
TATAC 2793170 0.6606478 13.366572 11
GCCTG 1241330 0.65787 6.5376 29
AGAAT 2737580 0.6566659 6.1772137 20
GCTCG 1232960 0.65343416 7.7436247 18
CGCCT 1172055 0.6486835 7.686453 28
CTGGG 1276350 0.64772487 6.265641 13
AACAG 1924760 0.6461405 7.6028004 10
CTTTG 2264665 0.6399459 9.076178 17
AGCTG 1588075 0.6369247 5.694262 2
CCCTG 1141375 0.6317034 6.4854875 2
GGTAT 2204025 0.6316272 6.3827915 22
CCCAT 1425205 0.62338805 6.1436634 3
GTGCA 1553285 0.6229716 12.118132 4
TCGCC 1109555 0.61409235 12.208096 27
CCCCA 1000720 0.61258525 7.8572507 2
ATAGT 2689630 0.6091624 15.121872 17
TGTGA 2101020 0.602108 7.328047 9
CTGCA 1436550 0.60168594 5.0400605 31
GTAAA 2494525 0.598364 11.927543 2
CTTTA 2623135 0.58580923 9.180343 18
TCTCT 1973095 0.5822631 5.090157 20
GCATT 1932540 0.57836866 8.80349 1
GTTAT 2704020 0.578247 13.10656 20
CCAGG 1026580 0.57621247 6.134617 11
CTGCC 1040555 0.5759037 6.120392 4
TTGGT 2111465 0.5713353 6.6043696 4
TGTAA 2489130 0.563752 11.676614 1
TAGGT 1964130 0.5628783 11.172586 9
TACTG 1870040 0.5596638 7.6891007 6
ATCCT 1788520 0.55898756 13.332464 8
AGTGC 1389530 0.5572948 11.964755 3
ATAAC 2217875 0.55558014 6.5130377 13
TAGTT 2597585 0.5554862 12.35814 18
GCTCA 1326125 0.5554354 7.217746 18
ATCCC 1261455 0.5517634 6.5108957 8
GCAGT 1354760 0.54334974 7.099072 10
TTTGA 2540000 0.5431718 5.921558 18
TACAG 1712355 0.5427592 8.715212 1
CGTAG 1352605 0.5424854 5.9229794 5
TGACT 1803055 0.5396165 7.8968706 13
TTCAG 1796875 0.537767 6.9819555 11
AGGTT 1873535 0.5369157 11.479995 10
CCTTG 1352490 0.5348674 10.627198 10
GGTTC 1410085 0.53398037 8.908448 9
GCGGT 1047040 0.5313541 5.3348703 10
GAATA 2213865 0.53104186 8.255922 15
ACAGT 1668955 0.52900285 7.995756 2
GCTTC 1331030 0.52638066 6.6404653 14
GTGAT 1827965 0.5238563 7.1583548 10
CATTG 1750280 0.52382207 9.178603 2
AAGCC 1170725 0.51932716 5.461156 8
TAAAC 2069105 0.51831305 12.285273 3
CAGTA 1610415 0.5104476 7.9827075 3
GCTGC 958055 0.5077422 6.2662325 3
ATATA 2821555 0.5050395 10.048645 10
ATTGA 2221835 0.50321364 8.3224125 12
ATTGG 1743935 0.49977508 7.4130087 3
CCTGC 891075 0.4931728 6.573495 30
TTGAC 1642440 0.49154785 7.8240314 12
GTATA 2116015 0.47924685 8.426238 15
CCCCG 617400 0.47821623 7.475381 10
CCGTG 895645 0.47466668 6.13592 11
CCGAC 807060 0.4730725 5.4474697 12
GCCAG 841600 0.47238445 6.276731 6
AGTTA 2027645 0.45923233 12.305704 19
TCCTT 1550735 0.45762408 8.153866 9
AACTT 1922860 0.45479983 9.113556 15
ATGGT 1583935 0.45392245 6.2899756 20
GTTCA 1467000 0.43904233 6.9857554 10
AGATT 1935070 0.43826544 8.401087 10
CCAGC 746175 0.43738368 6.539529 7
TATAG 1915310 0.4337901 8.390373 16
CCCGT 771525 0.42700687 5.8523655 3
GCCCT 762995 0.42228585 5.926931 10
TTTAT 2582170 0.41204777 6.464761 19
AATAG 1701085 0.40804082 8.24231 16
CGTGT 1069740 0.40509626 5.1569724 12
TCCCC 695710 0.40211022 5.925454 9
GATAA 1611830 0.38663116 6.200074 12
ACTTT 1731025 0.38657954 7.0770044 16
GTACT 1283380 0.38408872 7.5461497 5
ATTCT 1719515 0.38400906 5.3800006 23
TCCCT 925250 0.382123 5.0152597 1
AAGAT 1565290 0.3754676 5.5478883 20
AGTAC 1148235 0.36395204 7.6367297 4
TGATA 1597245 0.36175293 5.65608 11
TAACT 1492455 0.35299924 6.1078086 14
TAACA 1347220 0.33748007 5.609075 9
CATAA 1316300 0.3297346 5.7426686 7
CCCGA 550360 0.32260326 5.5386834 11
CCTAC 729425 0.31905225 6.5593624 10
Produced by FastQC (version 0.10.0)