FastQC Reportmar. 13 sept. 2011
ERR022486_1.fastq
Summary
![[PASS]](Icons/tick.png)
Basic Statistics- Per base sequence quality
- Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content- Per base GC content
![[WARNING]](Icons/warning.png)
Per sequence GC content- Per base N content
- Sequence Length Distribution
- Sequence Duplication Levels
- Overrepresented sequences
- Kmer Content
Basic Statistics
| Measure | Value |
|---|---|
| Filename | ERR022486_1.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 31504560 |
| Sequence length | 76 |
| %GC | 44 |
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 64433 | 0.20451959970239228 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TTTTT | 17091075 | 4.8419404 | 7.863731 | 2 |
| CACAC | 8645620 | 4.4589205 | 7.899114 | 7 |
| GTGTG | 7970515 | 4.3080115 | 5.1752267 | 6 |
| ACACA | 9699215 | 4.002724 | 7.926882 | 6 |
| TGTGT | 8864810 | 3.863193 | 5.047053 | 7 |
| AAAAA | 13551545 | 3.580792 | 6.9255157 | 2 |
| CTGGA | 4154955 | 2.2006643 | 5.9734254 | 1 |
| CTCCA | 4052920 | 2.1195982 | 5.825102 | 6 |
| TCCAG | 3880265 | 2.0421982 | 6.553185 | 7 |
| TCTGC | 3505250 | 1.8707143 | 5.232288 | 7 |
| GTTTG | 3822225 | 1.6656864 | 5.654764 | 1 |
| GTTTT | 4649465 | 1.6336782 | 6.4691815 | 1 |
| CTCAG | 3078985 | 1.6204815 | 5.1298175 | 1 |
| ATCCA | 2832505 | 1.1853368 | 5.6020374 | 6 |