FastQC Reportmar. 13 sept. 2011
ERR022486_2.fastq
Summary
![[PASS]](Icons/tick.png)
Basic Statistics- Per base sequence quality
- Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content- Per base GC content
- Per sequence GC content
- Per base N content
- Sequence Length Distribution
- Sequence Duplication Levels
![[WARNING]](Icons/warning.png)
Overrepresented sequences- Kmer Content
Basic Statistics
| Measure | Value |
|---|---|
| Filename | ERR022486_2.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 31504560 |
| Sequence length | 76 |
| %GC | 44 |
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 44325 | 0.14069391859464153 | No Hit |
| AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 42345 | 0.13440911410919562 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TTTTT | 15244720 | 4.3027563 | 7.4825525 | 2 |
| GTGTG | 8067190 | 4.2972207 | 5.4241505 | 6 |
| CACAC | 8281850 | 4.2583714 | 7.4245706 | 7 |
| AAAAA | 14754945 | 4.062385 | 7.8247914 | 2 |
| ACACA | 9266090 | 3.8689086 | 7.513649 | 6 |
| TGTGT | 8972115 | 3.867356 | 5.2740717 | 7 |
| CTGGA | 4100200 | 2.1549397 | 5.705727 | 1 |
| CTCCA | 4082465 | 2.1095772 | 5.776535 | 6 |
| TCCAG | 3880505 | 2.022273 | 6.3617907 | 7 |
| GTTTT | 4845500 | 1.690098 | 7.3602953 | 1 |
| GTTTG | 3900855 | 1.6814313 | 6.1718044 | 1 |
| ATCCA | 2854815 | 1.197917 | 5.5591817 | 6 |