FastQC Reportmar. 13 sept. 2011
ERR022488_1.fastq
Summary
![[PASS]](Icons/tick.png)
Basic Statistics- Per base sequence quality
- Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content- Per base GC content
![[WARNING]](Icons/warning.png)
Per sequence GC content- Per base N content
- Sequence Length Distribution
- Sequence Duplication Levels
- Overrepresented sequences
- Kmer Content
Basic Statistics
| Measure | Value |
|---|---|
| Filename | ERR022488_1.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 24920613 |
| Sequence length | 76 |
| %GC | 44 |
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 32060 | 0.12864852080484537 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TTTTT | 11296035 | 3.88962 | 6.6844645 | 2 |
| CACAC | 5443925 | 3.596398 | 6.2960324 | 7 |
| GTGTG | 5045165 | 3.434146 | 4.2426777 | 6 |
| AAAAA | 10030820 | 3.3843057 | 6.729543 | 2 |
| ACACA | 6212390 | 3.280501 | 6.5145264 | 6 |
| TGTGT | 5732100 | 3.1088731 | 4.288944 | 7 |
| CTGGA | 3316640 | 2.232146 | 6.107118 | 1 |
| CTCCA | 3269720 | 2.1688812 | 5.780912 | 6 |
| TCCAG | 3152575 | 2.1063967 | 6.617541 | 7 |
| TCTGC | 2791650 | 1.8728598 | 5.270806 | 7 |
| GTTTG | 2954010 | 1.6021427 | 5.267237 | 1 |
| GTTTT | 3677820 | 1.5893723 | 6.0336704 | 1 |
| ATCCA | 2292615 | 1.2155763 | 5.619658 | 6 |