FastQC Reportmar. 13 sept. 2011
ERR022488_2.fastq
Summary
![[PASS]](Icons/tick.png)
Basic Statistics- Per base sequence quality
- Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content- Per base GC content
![[WARNING]](Icons/warning.png)
Per sequence GC content- Per base N content
- Sequence Length Distribution
- Sequence Duplication Levels
- Overrepresented sequences
- Kmer Content
Basic Statistics
| Measure | Value |
|---|---|
| Filename | ERR022488_2.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 24920613 |
| Sequence length | 76 |
| %GC | 44 |
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 25674 | 0.10302314794583904 | No Hit |
| TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 25001 | 0.10032257232195693 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| AAAAA | 10895355 | 3.7596762 | 7.559924 | 2 |
| TTTTT | 10800315 | 3.7319453 | 6.824335 | 2 |
| GTGTG | 5160590 | 3.4567795 | 4.4046793 | 6 |
| CACAC | 5168310 | 3.431091 | 5.911703 | 7 |
| ACACA | 5906960 | 3.1529918 | 6.201835 | 6 |
| TGTGT | 5849445 | 3.1424072 | 4.4204016 | 7 |
| CTGGA | 3295260 | 2.2005258 | 5.861787 | 1 |
| CTCCA | 3249715 | 2.1579776 | 5.7746644 | 6 |
| TCCAG | 3129490 | 2.0839763 | 6.4257298 | 7 |
| GTTTT | 3765880 | 1.622519 | 6.8767314 | 1 |
| GTTTG | 3012425 | 1.6183186 | 5.812506 | 1 |
| ATCCA | 2288310 | 1.2217761 | 5.499171 | 6 |