set terminal png size 700,500 truecolor set output "SRR7062654.bam.stat-quals2.png" set grid xtics ytics y2tics back lc rgb "#cccccc" set multiplot set rmargin 0; set lmargin 0; set tmargin 0; set bmargin 0; set origin 0.1,0.1; set size 0.4,0.8 set yrange [0:50] set ylabel "Quality" set xlabel "Cycle (fwd reads)" plot '-' using 1:2:3 with filledcurve lt 1 lc rgb "#cccccc" t '25-75th percentile' , '-' using 1:2 with lines lc rgb "#000000" t 'Median', '-' using 1:2 with lines lt 1 t 'Mean' 1 31 34 2 31 34 3 31 34 4 35 37 5 35 37 6 35 37 7 35 37 8 35 37 9 37 39 10 37 39 11 37 39 12 37 39 13 37 39 14 38 41 15 38 41 16 38 41 17 38 41 18 38 41 19 38 41 20 37 41 21 37 41 22 37 41 23 37 41 24 37 41 25 37 41 26 37 41 27 37 41 28 36 40 29 36 40 30 36 40 31 36 41 32 37 41 33 36 41 34 36 41 35 36 41 36 36 41 37 36 41 38 36 41 39 36 40 40 36 40 41 36 41 42 36 41 43 35 41 44 35 40 45 35 40 46 35 40 47 35 40 48 35 40 49 35 40 50 35 40 51 35 40 52 34 40 53 34 40 54 34 40 55 34 40 56 34 40 57 33 40 58 33 40 59 33 40 60 33 39 61 33 39 62 32 39 63 32 39 64 32 39 65 32 39 66 31 38 67 31 38 68 31 38 69 31 37 70 31 37 71 30 37 72 30 36 73 30 36 74 30 36 75 30 36 76 29 35 77 29 35 78 30 35 79 30 35 80 29 35 81 29 35 82 29 35 83 29 35 84 29 35 85 29 35 86 28 35 87 27 35 88 27 35 89 26 35 90 25 34 end 1 31 2 33 3 34 4 37 5 37 6 37 7 37 8 37 9 39 10 39 11 39 12 39 13 39 14 40 15 40 16 40 17 40 18 40 19 40 20 40 21 40 22 40 23 40 24 40 25 40 26 39 27 39 28 39 29 39 30 39 31 39 32 39 33 39 34 39 35 39 36 39 37 39 38 39 39 39 40 39 41 39 42 39 43 39 44 39 45 39 46 39 47 39 48 38 49 38 50 38 51 38 52 38 53 38 54 38 55 38 56 37 57 37 58 37 59 37 60 36 61 36 62 36 63 36 64 35 65 35 66 35 67 35 68 35 69 35 70 35 71 34 72 34 73 34 74 34 75 34 76 33 77 34 78 34 79 34 80 34 81 34 82 34 83 34 84 33 85 33 86 33 87 33 88 33 89 33 90 32 end 1 32.83 2 33.14 3 33.23 4 36.85 5 36.75 6 36.75 7 36.72 8 36.68 9 38.29 10 38.26 11 38.24 12 38.21 13 38.19 14 39.46 15 39.41 16 39.36 17 39.31 18 39.24 19 39.23 20 39.18 21 39.14 22 39.06 23 39.00 24 38.93 25 38.86 26 38.69 27 38.59 28 38.46 29 38.35 30 38.23 31 38.50 32 38.54 33 38.51 34 38.47 35 38.42 36 38.34 37 38.27 38 38.17 39 38.08 40 38.12 41 38.13 42 38.09 43 38.04 44 37.99 45 37.92 46 37.80 47 37.72 48 37.58 49 37.48 50 37.34 51 37.20 52 37.03 53 36.80 54 36.67 55 36.52 56 36.31 57 36.18 58 35.95 59 35.77 60 35.60 61 35.39 62 35.16 63 34.94 64 34.71 65 34.48 66 34.21 67 33.97 68 33.69 69 33.45 70 33.20 71 32.95 72 32.67 73 32.41 74 32.17 75 31.94 76 31.36 77 31.53 78 31.56 79 31.43 80 31.23 81 30.98 82 30.70 83 30.43 84 30.11 85 29.77 86 29.42 87 29.01 88 28.57 89 28.09 90 27.45 end set origin 0.55,0.1 set size 0.4,0.8 unset ytics set y2tics mirror set yrange [0:50] unset ylabel set xlabel "Cycle (rev reads)" set label "SRR7062654.bam.stat" at screen 0.5,0.95 center noenhanced plot '-' using 1:2:3 with filledcurve lt 1 lc rgb "#cccccc" t '25-75th percentile' , '-' using 1:2 with lines lc rgb "#000000" t 'Median', '-' using 1:2 with lines lt 2 t 'Mean' 1 31 34 2 31 34 3 31 34 4 35 37 5 35 37 6 35 37 7 35 37 8 35 37 9 35 39 10 35 39 11 35 39 12 35 39 13 35 39 14 36 41 15 36 41 16 36 41 17 36 41 18 36 41 19 36 41 20 36 41 21 36 41 22 36 40 23 36 40 24 35 40 25 35 40 26 35 40 27 35 40 28 34 40 29 34 40 30 35 40 31 34 40 32 35 40 33 35 40 34 35 40 35 34 40 36 34 40 37 34 40 38 34 40 39 34 40 40 34 40 41 33 40 42 33 40 43 33 40 44 33 40 45 33 40 46 33 40 47 33 40 48 33 40 49 33 40 50 33 40 51 32 39 52 32 39 53 33 40 54 32 40 55 32 40 56 32 40 57 32 40 58 31 39 59 31 39 60 31 39 61 31 39 62 31 39 63 30 39 64 30 38 65 30 38 66 29 38 67 29 38 68 29 37 69 28 37 70 28 37 71 28 36 72 27 36 73 27 36 74 26 36 75 26 35 76 26 35 77 26 35 78 25 35 79 25 35 80 24 35 81 23 35 82 21 34 83 20 34 84 18 34 85 17 34 86 7 34 87 2 34 88 2 34 89 2 34 90 2 34 end 1 31 2 31 3 31 4 37 5 37 6 37 7 37 8 37 9 39 10 39 11 39 12 38 13 38 14 39 15 39 16 39 17 39 18 39 19 39 20 39 21 39 22 39 23 39 24 39 25 39 26 39 27 38 28 38 29 38 30 38 31 38 32 38 33 38 34 38 35 38 36 38 37 38 38 38 39 38 40 38 41 38 42 38 43 38 44 38 45 38 46 38 47 38 48 38 49 38 50 38 51 37 52 37 53 37 54 37 55 37 56 37 57 37 58 36 59 36 60 36 61 35 62 35 63 35 64 35 65 35 66 35 67 34 68 34 69 34 70 34 71 34 72 34 73 33 74 33 75 33 76 33 77 33 78 32 79 32 80 32 81 32 82 31 83 31 84 31 85 31 86 31 87 31 88 30 89 30 90 30 end 1 31.98 2 32.15 3 32.22 4 35.78 5 35.82 6 35.79 7 35.77 8 35.80 9 37.13 10 37.17 11 37.15 12 37.11 13 37.05 14 38.13 15 38.06 16 38.02 17 37.94 18 37.90 19 37.97 20 37.84 21 37.81 22 37.73 23 37.65 24 37.56 25 37.42 26 37.37 27 37.25 28 37.14 29 37.03 30 37.26 31 36.99 32 37.14 33 37.15 34 37.10 35 37.03 36 36.95 37 36.86 38 36.76 39 36.64 40 36.51 41 36.40 42 36.26 43 36.17 44 36.27 45 36.30 46 36.29 47 36.17 48 36.00 49 35.85 50 35.65 51 34.99 52 35.22 53 35.32 54 35.24 55 35.10 56 34.90 57 34.71 58 34.43 59 34.22 60 33.96 61 33.71 62 33.43 63 33.19 64 32.90 65 32.61 66 32.27 67 31.95 68 31.62 69 31.29 70 30.96 71 30.66 72 30.30 73 30.01 74 29.67 75 29.30 76 28.94 77 28.60 78 28.26 79 27.89 80 27.48 81 27.09 82 26.70 83 26.31 84 25.87 85 25.43 86 24.94 87 24.41 88 23.83 89 23.22 90 22.48 end