set terminal png size 700,500 truecolor set output "SRR7062654.md.recal.bam.stat-quals2.png" set grid xtics ytics y2tics back lc rgb "#cccccc" set multiplot set rmargin 0; set lmargin 0; set tmargin 0; set bmargin 0; set origin 0.1,0.1; set size 0.4,0.8 set yrange [0:50] set ylabel "Quality" set xlabel "Cycle (fwd reads)" plot '-' using 1:2:3 with filledcurve lt 1 lc rgb "#cccccc" t '25-75th percentile' , '-' using 1:2 with lines lc rgb "#000000" t 'Median', '-' using 1:2 with lines lt 1 t 'Mean' 1 29 30 2 29 31 3 30 32 4 31 33 5 31 33 6 30 32 7 30 32 8 30 32 9 31 33 10 30 33 11 30 32 12 30 32 13 30 32 14 30 33 15 30 32 16 30 33 17 30 33 18 30 33 19 30 32 20 30 32 21 30 33 22 30 32 23 30 32 24 30 33 25 30 33 26 30 32 27 30 33 28 30 32 29 30 33 30 30 32 31 30 33 32 30 33 33 30 33 34 30 33 35 30 33 36 30 33 37 30 33 38 30 33 39 30 33 40 30 33 41 30 32 42 30 33 43 30 33 44 30 33 45 30 33 46 30 33 47 30 33 48 30 33 49 30 33 50 30 33 51 30 33 52 30 33 53 30 33 54 30 33 55 30 32 56 30 32 57 30 32 58 30 33 59 30 33 60 30 33 61 30 33 62 29 32 63 29 32 64 30 32 65 29 33 66 29 32 67 29 33 68 29 32 69 29 32 70 29 33 71 29 33 72 29 32 73 29 32 74 29 32 75 29 32 76 29 32 77 29 32 78 29 32 79 29 32 80 29 32 81 29 32 82 29 32 83 29 32 84 29 32 85 29 32 86 29 32 87 29 32 88 28 32 89 28 32 90 27 31 end 1 29 2 30 3 31 4 32 5 32 6 31 7 31 8 31 9 32 10 32 11 31 12 31 13 32 14 32 15 31 16 32 17 32 18 32 19 32 20 31 21 32 22 31 23 31 24 32 25 32 26 31 27 32 28 32 29 32 30 31 31 32 32 32 33 32 34 32 35 32 36 32 37 31 38 32 39 32 40 32 41 32 42 32 43 32 44 32 45 32 46 32 47 32 48 31 49 32 50 32 51 32 52 31 53 31 54 31 55 31 56 31 57 31 58 31 59 31 60 31 61 31 62 31 63 31 64 31 65 31 66 31 67 31 68 31 69 31 70 31 71 31 72 31 73 31 74 31 75 31 76 31 77 31 78 31 79 31 80 31 81 31 82 31 83 31 84 31 85 31 86 31 87 31 88 31 89 31 90 30 end 1 30.05 2 30.77 3 31.69 4 32.75 5 32.71 6 32.06 7 32.03 8 32.02 9 32.55 10 32.34 11 31.73 12 31.74 13 32.08 14 32.19 15 31.87 16 32.32 17 32.30 18 32.29 19 32.10 20 32.03 21 32.40 22 31.98 23 32.04 24 32.30 25 32.40 26 31.93 27 32.03 28 31.98 29 32.13 30 31.90 31 32.26 32 32.26 33 32.25 34 32.21 35 32.21 36 32.11 37 31.91 38 32.13 39 32.10 40 31.95 41 31.91 42 32.05 43 32.03 44 32.21 45 31.98 46 31.94 47 31.88 48 31.75 49 31.79 50 31.80 51 31.77 52 31.59 53 31.44 54 31.67 55 31.34 56 31.33 57 31.30 58 31.27 59 31.32 60 31.37 61 31.09 62 30.96 63 30.82 64 30.83 65 30.81 66 30.66 67 30.74 68 30.37 69 30.38 70 30.45 71 30.39 72 30.06 73 29.88 74 29.83 75 29.63 76 29.56 77 29.57 78 29.78 79 29.40 80 29.37 81 29.32 82 29.20 83 28.84 84 28.86 85 28.53 86 28.32 87 27.99 88 27.51 89 26.94 90 25.72 end set origin 0.55,0.1 set size 0.4,0.8 unset ytics set y2tics mirror set yrange [0:50] unset ylabel set xlabel "Cycle (rev reads)" set label "SRR7062654.md.recal.bam.stat" at screen 0.5,0.95 center noenhanced plot '-' using 1:2:3 with filledcurve lt 1 lc rgb "#cccccc" t '25-75th percentile' , '-' using 1:2 with lines lc rgb "#000000" t 'Median', '-' using 1:2 with lines lt 2 t 'Mean' 1 27 28 2 28 31 3 29 32 4 30 32 5 30 32 6 29 31 7 30 32 8 30 32 9 29 31 10 30 32 11 30 32 12 30 32 13 30 32 14 29 32 15 29 32 16 30 32 17 29 32 18 29 32 19 30 32 20 30 32 21 30 33 22 30 33 23 30 32 24 30 33 25 29 32 26 30 32 27 30 32 28 30 32 29 30 33 30 30 32 31 27 31 32 30 33 33 30 32 34 30 32 35 30 32 36 30 33 37 30 32 38 30 33 39 30 33 40 30 33 41 30 33 42 30 33 43 30 33 44 30 33 45 30 33 46 30 32 47 30 32 48 30 32 49 30 32 50 30 33 51 30 32 52 30 33 53 30 33 54 30 33 55 30 33 56 29 32 57 29 33 58 29 33 59 29 33 60 29 32 61 29 32 62 29 32 63 29 32 64 29 33 65 29 33 66 29 32 67 29 32 68 29 32 69 28 32 70 28 32 71 28 32 72 28 32 73 28 32 74 28 32 75 27 32 76 27 32 77 27 32 78 27 32 79 26 32 80 26 32 81 26 32 82 25 32 83 24 32 84 23 32 85 21 32 86 15 32 87 2 31 88 2 32 89 2 32 90 2 31 end 1 27 2 30 3 31 4 31 5 31 6 30 7 31 8 31 9 30 10 31 11 31 12 31 13 31 14 31 15 31 16 31 17 31 18 31 19 31 20 31 21 32 22 32 23 31 24 32 25 31 26 31 27 31 28 31 29 32 30 31 31 30 32 32 33 31 34 31 35 31 36 32 37 31 38 31 39 31 40 31 41 31 42 31 43 31 44 31 45 31 46 31 47 31 48 31 49 31 50 31 51 31 52 31 53 31 54 31 55 31 56 31 57 31 58 31 59 31 60 31 61 31 62 31 63 31 64 31 65 31 66 31 67 31 68 31 69 31 70 31 71 31 72 30 73 31 74 30 75 30 76 31 77 30 78 30 79 30 80 30 81 30 82 30 83 30 84 30 85 30 86 30 87 30 88 30 89 30 90 29 end 1 27.82 2 30.22 3 30.96 4 31.55 5 31.59 6 30.71 7 31.27 8 31.58 9 30.35 10 31.25 11 31.15 12 31.22 13 31.26 14 31.10 15 31.07 16 31.23 17 30.79 18 31.09 19 31.55 20 31.34 21 31.76 22 31.72 23 31.39 24 31.67 25 30.97 26 31.49 27 31.18 28 31.35 29 31.59 30 31.41 31 29.25 32 31.62 33 31.35 34 31.11 35 31.36 36 31.53 37 31.23 38 31.40 39 31.40 40 31.34 41 31.47 42 31.27 43 31.21 44 31.49 45 31.34 46 31.29 47 31.21 48 31.07 49 31.02 50 31.06 51 30.79 52 30.99 53 30.91 54 30.85 55 30.82 56 30.43 57 30.57 58 30.56 59 30.35 60 30.05 61 29.95 62 29.94 63 29.76 64 29.66 65 29.48 66 29.16 67 29.02 68 28.97 69 28.71 70 28.61 71 28.49 72 28.03 73 27.98 74 27.60 75 27.40 76 27.44 77 27.15 78 26.76 79 26.63 80 26.22 81 26.16 82 25.95 83 25.53 84 25.12 85 24.71 86 24.36 87 23.71 88 23.26 89 22.51 90 21.33 end