Inputs and parameters

Parameters

Run functional and taxonomic annotations Yes
Run MEGAN Yes
Run annotations with COGs Yes
Build submission files Yes
Run blast against your own fasta file No

Annotation

Result table

Prokka Fasta files	Prokka annotation	Diamond fosmid against NR	Diamond fosmid against Uniprot	Diamond ORFs against NR	Diamond ORFs against Uniprot	COGs annotation
DNA - PROT	GFF	TSV	TSV	TSV	TSV	RPSBLAST

Result table by fosmid

Sample	Length	GC%	A	T	G	C	N	Prokka	GenBank	Annotated ORFs against Uniprot		Annotated ORFs against NR		MEGAN	COGs
BifidoAdolescentis.s2	35132	57.73	7558	7294	10124	10156	0	Fasta	GBK	21 / 38		32 / 38			DNA replication, recombination, and repair
BifidoAdolescentis.s6	42067	59.58	8598	8404	12932	12133	0	Fasta	GBK	19 / 35		33 / 35			Carbohydrate transport and metabolism

ORF	HSP	Max coverage	Best HSP (max. of %iden * coverage)
ORF	HSP	Max coverage	%iden.	Coverage	Species
ORF1	698	99.5%	88.6	99.5%	Bifidobacterium pseudolongum
ORF2	920	99.5%	85.9	99.5%	Bifidobacterium dentium
ORF3	1000	99.7%	99.4	98.3%	Bifidobacterium adolescentis
ORF4	31	99.3%	98.5	99.3%	Bifidobacterium adolescentis
ORF5	995	99.5%	83.4	99.5%	Bifidobacterium pseudocatenulatum
ORF6	993	99.7%	99.2	99.7%	Bifidobacterium adolescentis
ORF7	1000	99.7%	94.5	99.7%	Bifidobacterium dentium
ORF8	42	99.8%	99.6	99.8%	Bifidobacterium adolescentis
ORF9	1000	99.8%	99.8	99.8%	Bifidobacterium adolescentis
ORF10	21	98.9%	98.9	98.9%	Bifidobacterium adolescentis
ORF11	251	99.7%	99.0	99.7%	Bifidobacterium adolescentis
ORF12	998	99.6%	99.6	99.6%	Bifidobacterium adolescentis
ORF13	1000	99.9%	99.6	99.9%	Bifidobacterium adolescentis
ORF14	1	96.4%	71.4	96.4%	Bifidobacterium pseudocatenulatum
ORF16	558	99.8%	99.6	99.8%	Bifidobacterium adolescentis
ORF17	35	99.8%	99.7	99.8%	Bifidobacterium adolescentis
ORF18	22	99.1%	65.7	99.1%	Bifidobacterium pseudocatenulatum
ORF19	1000	99.9%	99.8	99.9%	Bifidobacterium adolescentis
ORF20	270	99.1%	90.4	99.1%	Bifidobacterium pseudocatenulatum
ORF21	208	99.6%	99.6	99.6%	Bifidobacterium adolescentis
ORF22	5	99.2%	97.5	99.2%	Bifidobacterium adolescentis
ORF23	404	84.8%	83.8	80.3%	Bifidobacterium pseudocatenulatum
ORF24	361	97.3%	93.8	97.3%	Bifidobacterium pseudocatenulatum
ORF25	1	68.0%	60.8	68.0%	Bifidobacterium thermophilum RBL67
ORF31	321	99.6%	99.3	99.6%	Bifidobacterium adolescentis
ORF32	993	99.7%	88.8	99.7%	Bifidobacterium pseudocatenulatum
ORF33	342	98.2%	82.1	96.0%	Bifidobacterium dentium ATCC 27679
ORF34	1000	99.7%	99.2	99.7%	Bifidobacterium adolescentis
ORF35	953	99.7%	86.9	98.9%	Bifidobacterium pseudocatenulatum
ORF36	1000	99.9%	99.7	99.9%	Bifidobacterium adolescentis
ORF37	966	99.7%	93.2	99.5%	Bifidobacteriaceae bacterium NR047

ORF	HSP	Max coverage	Best HSP (max. of %iden * coverage)
ORF	HSP	Max coverage	%iden.	Coverage	Species
ORF1	26	98.8%	98.8	98.8%	Bifidobacterium adolescentis
ORF2	398	99.6%	99.2	99.6%	Bifidobacterium adolescentis
ORF3	6	99.2%	99.2	99.2%	Bifidobacterium adolescentis
ORF4	297	99.6%	97.9	99.6%	Bifidobacterium adolescentis
ORF5	276	96.4%	97.7	95.1%	Bifidobacterium adolescentis
ORF6	732	99.7%	98.8	99.7%	Bifidobacterium adolescentis
ORF7	693	99.6%	95.8	99.6%	Bifidobacterium pseudocatenulatum
ORF8	732	99.6%	96.4	99.6%	Bifidobacterium adolescentis
ORF9	83	99.5%	99.5	99.5%	Bifidobacterium adolescentis
ORF10	1000	99.8%	99.7	99.8%	Bifidobacterium adolescentis
ORF11	988	99.9%	99.7	99.9%	Bifidobacterium adolescentis
ORF12	1000	99.8%	85.9	99.8%	Bifidobacterium adolescentis
ORF13	119	99.6%	87.2	98.5%	Bifidobacterium adolescentis
ORF14	40	99.6%	99.3	99.6%	Bifidobacterium adolescentis
ORF15	583	99.7%	95.9	98.8%	Bifidobacterium adolescentis
ORF17	16	99.7%	96.5	99.7%	Bifidobacterium adolescentis
ORF18	999	99.7%	97.6	99.7%	Bifidobacterium adolescentis
ORF19	3	99.4%	97.1	99.4%	Bifidobacterium adolescentis
ORF20	182	99.7%	97.9	99.7%	Bifidobacterium adolescentis
ORF21	707	99.9%	97.7	99.9%	Bifidobacterium adolescentis
ORF22	1000	93.8%	99.0	93.8%	Bifidobacterium
ORF23	249	99.6%	96.9	99.6%	Bifidobacterium adolescentis
ORF24	1000	99.7%	97.4	99.7%	Bifidobacterium adolescentis
ORF25	389	99.4%	99.4	99.4%	Bifidobacterium adolescentis
ORF26	58	98.9%	91.1	98.9%	Bifidobacterium dentium ATCC 27679
ORF27	516	99.6%	98.8	99.6%	Bifidobacterium adolescentis
ORF29	755	99.6%	98.7	99.6%	Bifidobacterium adolescentis
ORF30	515	99.7%	97.6	99.7%	Bifidobacterium adolescentis
ORF31	919	99.7%	99.7	99.7%	Bifidobacterium adolescentis
ORF32	1	54.9%	97.8	54.9%	Bifidobacterium adolescentis
ORF33	28	99.6%	98.7	99.6%	Bifidobacterium adolescentis
ORF34	998	99.8%	99.8	99.8%	Bifidobacterium adolescentis

ORF	HSP	Max coverage	Best HSP (max. of %iden * coverage)
ORF	HSP	Max coverage	%iden.	Coverage	Species
ORF1	168	99.5%	99.5	99.5%	Bifidobacterium adolescentis
ORF2	499	99.5%	100.0	99.5%	Bifidobacterium adolescentis
ORF3	707	99.7%	100.0	99.7%	Bifidobacterium adolescentis
ORF5	607	99.5%	100.0	99.5%	Bifidobacterium adolescentis
ORF6	504	99.5%	50.3	98.7%	Frankia casuarinae
ORF7	51	99.7%	57.8	98.7%	Mycobacterium bovis
ORF9	471	99.8%	85.9	99.8%	Bifidobacterium longum
ORF11	13	74.7%	33.0	73.7%	Agrobacterium fabrum
ORF12	53	96.9%	49.4	96.9%	Mycobacterium tuberculosis
ORF13	622	68.2%	86.2	65.2%	Bifidobacterium longum
ORF16	20	93.5%	35.7	91.6%	Candida albicans
ORF19	25	94.4%	38.3	94.4%	Mycobacterium leprae
ORF20	4	94.0%	51.8	94.0%	Streptomyces coelicolor
ORF23	3	78.5%	46.3	78.5%	Mycobacterium tuberculosis
ORF24	8	93.3%	47.4	89.3%	Corynebacterium efficiens
ORF32	587	99.7%	100.0	99.7%	Bifidobacterium adolescentis
ORF34	699	99.7%	69.9	99.5%	Bifidobacterium longum
ORF35	250	99.7%	100.0	99.7%	Bifidobacterium adolescentis
ORF36	67	99.0%	51.8	97.6%	Mycobacterium leprae
ORF37	48	99.2%	58.5	98.6%	Renibacterium salmoninarum

ORF	HSP	Max coverage	Best HSP (max. of %iden * coverage)
ORF	HSP	Max coverage	%iden.	Coverage	Species
ORF2	12	89.2%	35.6	85.4%	Staphylococcus epidermidis
ORF4	17	88.9%	31.5	78.7%	Rhodospirillum centenum
ORF6	103	98.8%	31.1	98.5%	Escherichia coli
ORF7	10	96.8%	40.4	95.4%	Thermoanaerobacterium thermosulfurigenes
ORF8	6	99.3%	39.2	95.4%	Thermoanaerobacterium thermosulfurigenes
ORF10	52	97.9%	33.7	94.8%	Parageobacillus thermoglucosidasius
ORF11	7	97.2%	43.0	97.2%	Mycobacterium bovis
ORF12	40	84.9%	36.5	84.3%	Thermotoga maritima
ORF15	100	97.1%	28.4	96.5%	Vibrio vulnificus
ORF21	20	73.0%	46.0	73.0%	Pseudomonas aeruginosa
ORF22	1000	99.8%	100.0	99.8%	Bifidobacterium adolescentis
ORF23	47	99.6%	100.0	99.6%	Bifidobacterium adolescentis
ORF24	593	97.7%	40.5	96.2%	Clostridium beijerinckii
ORF25	1	73.2%	45.9	73.2%	Streptomyces coelicolor
ORF27	1	74.3%	29.3	74.3%	Bacillus subtilis
ORF29	4	91.9%	30.2	91.9%	Escherichia coli
ORF30	1	73.6%	37.4	73.6%	Butyrivibrio fibrisolvens
ORF31	27	80.8%	45.1	80.8%	Mycobacterium bovis
ORF34	291	99.8%	100.0	99.8%	Bifidobacterium adolescentis

COGs category	ORFs	COGs (Best hit)
DNA replication, recombination, and repair	ORF1	COG0817, RuvC, Holliday junction resolvasome, endonuclease subunit
	ORF2	COG0632, RuvA, Holliday junction resolvasome, DNA-binding subunit
	ORF3	COG2255, RuvB, Holliday junction resolvasome, helicase subunit
	ORF19	COG4581, Superfamily II RNA helicase
Carbohydrate transport and metabolism	ORF16	COG4284, UDP-glucose pyrophosphorylase
	ORF36	COG0021, TktA, Transketolase
	ORF37	COG0176, MipB, Transaldolase
Energy production and conversion	ORF6	COG0045, SucC, Succinyl-CoA synthetase, beta subunit
Energy production and conversion	ORF7	COG0074, SucD, Succinyl-CoA synthetase, alpha subunit
Transcription	ORF23	COG0789, SoxR, Predicted transcriptional regulators
Transcription	ORF35	COG1420, HrcA, Transcriptional regulator of heat shock gene
General function prediction only	ORF13	COG1160, Predicted GTPases
General function prediction only	ORF21	COG0546, Gph, Predicted phosphatases
Translation, ribosomalstructure and biogenesis	ORF12	COG1187, RsuA, 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases
Nucleotide transportand metabolism	ORF9	COG0138, PurH, AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)
Carbohydrate transportand metabolism	ORF11	COG0580, GlpF, Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)
Intracellular trafficking and secretion	ORF4	COG1862, YajC, Preprotein translocase subunit YajC
Posttranslational modification, protein turnover,chaperones	ORF34	COG0484, DnaJ, DnaJ-class molecular chaperone with C-terminal Zn finger domain
Function unknown	ORF33	COG3001, Uncharacterized protein conserved in bacteria
Amino acid transport and metabolism	ORF38	COG0436, Aspartate/tyrosine/aromatic aminotransferase
Nucleotide transport and metabolism	ORF5	COG0503, Apt, Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins
Lipid transport and metabolism	ORF32	COG1968, BacA, Undecaprenyl pyrophosphate phosphatase
Signal transduction mechanisms	ORF24	COG1716, FOG: FHA domain

COGs category	ORFs	COGs (Best hit)
Carbohydrate transport and metabolism	ORF5	COG1653, UgpB, ABC-type sugar transport system, periplasmic component
	ORF7	COG1175, UgpA, ABC-type sugar transport systems, permease components
	ORF8	COG0395, UgpE, ABC-type sugar transport system, permease component
	ORF10	COG0366, AmyA, Glycosidases
	ORF11	COG1640, MalQ, 4-alpha-glucanotransferase
	ORF12	COG1523, PulA, Type II secretory pathway, pullulanase PulA and related glycosidases
General function prediction only	ORF4	COG0406, phoE, Broad specificity phosphatase PhoE and related phosphatases
	ORF13	COG1011, Predicted hydrolase (HAD superfamily)
	ORF20	COG0730, Predicted permeases
	ORF27	COG0637, Predicted phosphatase/phosphohexomutase
	ORF31	COG0313, Predicted methyltransferases
Transcription	ORF6	COG1609, PurR, Transcriptional regulators
	ORF15	COG1609, PurR, Transcriptional regulators
	ORF25	COG0789, SoxR, Predicted transcriptional regulators
Posttranslational modification, protein turnover, chaperones	ORF22	COG0443, DnaK, Molecular chaperone
	ORF23	COG0576, GrpE, Molecular chaperone GrpE (heat shock protein)
Translation, ribosomal structure and biogenesis	ORF34	COG0143, MetG, Methionyl-tRNA synthetase
Function unknown	ORF29	COG0586, DedA, Uncharacterized membrane-associated protein
Cell envelope biogenesis, outer membrane	ORF18	COG3764, SrtA, Sortase (surface protein transpeptidase)
Lipid metabolism	ORF30	COG0657, Aes, Esterase/lipase
Energy production and conversion	ORF2	COG0778, NfnB, Nitroreductase
Inorganic ion transport and metabolism	ORF21	COG1785, PhoA, Alkaline phosphatase
Posttranslational modification, protein turnover,chaperones	ORF24	COG0484, DnaJ, DnaJ-class molecular chaperone with C-terminal Zn finger domain

Inputs and parameters

Parameters

Annotation

Result table

Result table by fosmid

Fosmid browser